| Lipids of cultured hepatoma cells. VI. Glycerolipid and monoenoic fatty acid biosynthesis in minimal deviation hepatoma 7288C-1. | |
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MedLine Citation:
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PMID: 165343 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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1-14C-Acetic, 1-14C-palmitic, or 1-14C-stearic acid was incubated with minimal deviation hepatoma 7288C cells grown in culture to assess: de novo fatty acid synthesis, oxidation, desaturation, and elongation of saturated fatty acids, as well as the ability of media fatty acids to serve as precursors of cellular glycerolipids. Distribution of radioactivity in the individual lipid classes and the various fatty acids of triglyceride, phosphatidyl choline, and phosphatidyl ethanolamine was determined. The radioactivity among the monoenoic acid isomers derived from triglyceride, phosphatidyl choline, and phosphatidyl ethanolamine was analyzed by reductive ozonolysis. Only small amounts of the labeled substrates were oxidized to carbon dioxide. Except for labeled stearic acid, which also was incorporated heavily into phosphatidyl inositol and phosphatidyl serine, most radioactivity was recovered in triglyceride, phosphatidyl choline, and phosphatidyl ethanolamine. Synthesis of cholesterol and long chain fatty acids from labeled acetic acid demonstrated that these cells can perform de novo synthesis of fatty acids and cholesterol. Both labeled palmitic and stearic acids were desaturated to the corresponding delta9 monoenes, and palmitic and palmitoleic acids were elongated. The nexadecenoic acid fraction isolated from triglyceride, phosphatidyl choline, and phosphatidyl ethanolamine, when acetic or palmitic acid was the labeled substrate, showed that greater than 70 percent of the labeled acids were the delta9 isomer. Radioactivity of the octadecenoic acid fraction derived from labeled acetic or palmitic acids was nearly equally divided between the delta9 isomer, oleic acid, and the delta11 isomer, vaccenic acid. Desaturation of labeled stearic acid produced only oleic acid. These data demonstrate that the biosynthesis of vaccenic acid in these cultured neoplastic cells proceeds via the elongation of palmitoleic acid. The relatively high level of vaccenic acid synthesis in these cells suggests that the reported elevation of "oleic acid" in many neoplasms may result from increased concentration of vaccenic acid. |
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Authors:
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R D Wiegand; R Wood |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Lipids Volume: 10 ISSN: 0024-4201 ISO Abbreviation: Lipids Publication Date: 1975 Mar |
Date Detail:
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Created Date: 1975-07-18 Completed Date: 1975-07-18 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0060450 Medline TA: Lipids Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 194-201 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetates
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metabolism Animals Carcinoma, Hepatocellular / metabolism* Cell Line Cholesterol / biosynthesis Fatty Acids, Unsaturated / biosynthesis* Lipids / biosynthesis* Liver Neoplasms Neoplasms, Experimental / metabolism Palmitic Acids / metabolism Phosphatidylcholines / biosynthesis Phosphatidylethanolamines / biosynthesis Phosphatidylinositols / biosynthesis Phosphatidylserines / biosynthesis Rats Stearic Acids / metabolism Time Factors Triglycerides / biosynthesis |
| Chemical | |
Reg. No./Substance:
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0/Acetates; 0/Fatty Acids, Unsaturated; 0/Lipids; 0/Palmitic Acids; 0/Phosphatidylcholines; 0/Phosphatidylethanolamines; 0/Phosphatidylinositols; 0/Phosphatidylserines; 0/Stearic Acids; 0/Triglycerides; 57-88-5/Cholesterol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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