Document Detail

Lipidomics of cellular and secreted phospholipids from differentiated human fetal type II alveolar epithelial cells.
MedLine Citation:
PMID:  16513897     Owner:  NLM     Status:  MEDLINE    
Maturation of fetal alveolar type II epithelial cells in utero is characterized by specific changes to lung surfactant phospholipids. Here, we quantified the effects of hormonal differentiation in vitro on the molecular specificity of cellular and secreted phospholipids from human fetal type II epithelial cells using electrospray ionization mass spectrometry. Differentiation, assessed by morphology and changes in gene expression, was accompanied by restricted and specific modifications to cell phospholipids, principally enrichments of shorter chain species of phosphatidylcholine (PC) and phosphatidylinositol, that were not observed in fetal lung fibroblasts. Treatment of differentiated epithelial cells with secretagogues stimulated the secretion of functional surfactant-containing surfactant proteins B and C (SP-B and SP-C). Secreted material was further enriched in this same set of phospholipid species but was characterized by increased contents of short-chain monounsaturated and disaturated species other than dipalmitoyl PC (PC16:0/16:0), principally palmitoylmyristoyl PC (PC16:0/14:0) and palmitoylpalmitoleoyl PC (PC16:0/16:1). Mixtures of these PC molecular species, phosphatidylglycerol, and SP-B and SP-C were functionally active and rapidly generated low surface tension on compression in a pulsating bubble surfactometer. These results suggest that hormonally differentiated human fetal type II cells do not select the molecular composition of surfactant phospholipid on the basis of saturation but, more likely, on the basis of acyl chain length.
Anthony D Postle; Linda W Gonzales; Wolfgang Bernhard; Graeme T Clark; Marye H Godinez; Rodolfo I Godinez; Philip L Ballard
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2006-03-02
Journal Detail:
Title:  Journal of lipid research     Volume:  47     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2006-05-23     Completed Date:  2006-07-25     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1322-31     Citation Subset:  IM    
Division of Infection, Inflammation, and Repair, School of Medicine, University of Southampton, Southampton, UK.
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MeSH Terms
1,2-Dipalmitoylphosphatidylcholine / metabolism
1-Methyl-3-isobutylxanthine / pharmacology
8-Bromo Cyclic Adenosine Monophosphate / metabolism
Cell Differentiation
Cells, Cultured
Dexamethasone / pharmacology
Dimyristoylphosphatidylcholine / metabolism
Epithelial Cells / cytology,  drug effects,  metabolism*
Lipid Metabolism*
Phosphatidylcholines / metabolism
Phospholipids / chemistry,  metabolism*
Pulmonary Alveoli / cytology,  drug effects,  metabolism*
Pulmonary Surfactant-Associated Proteins / metabolism
Pulmonary Surfactants / chemistry
Surface Tension
Grant Support
055490//Wellcome Trust; 457405//Wellcome Trust; HL-19737/HL/NHLBI NIH HHS; HL-56401/HL/NHLBI NIH HHS
Reg. No./Substance:
0/Phosphatidylcholines; 0/Phospholipids; 0/Pulmonary Surfactant-Associated Proteins; 0/Pulmonary Surfactants; 13699-48-4/Dimyristoylphosphatidylcholine; 23583-48-4/8-Bromo Cyclic Adenosine Monophosphate; 2644-64-6/1,2-Dipalmitoylphosphatidylcholine; 28822-58-4/1-Methyl-3-isobutylxanthine; 50-02-2/Dexamethasone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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