| Lipidomic analysis of the liver identifies changes of major lipid species in adiponectin deficient mice. | |
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MedLine Citation:
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PMID: 22465357 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Adiponectin protects from hepatic fat storage but surprisingly adiponectin deficient mice (APN-/-) fed a standard chow do not develop liver steatosis. This indicates that other pathways might be activated to compensate for adiponectin deficiency. An unbiased and comprehensive screen of major lipid classes was performed to identify hepatic alterations in these mice. APN-/- mice had decreased hepatic cholesterol while active SREBP2 and systemic cholesterol were not altered. Upregulation of cytochromes for bile acid synthesis suggests enhanced biliary cholesterol excretion. Analysis of 37 individual fatty acid species showed reduced stearate whereas total fatty acids were increased. Triglycerides and phospholipids were equal suggesting elevation of unesterified free fatty acids. Elongase activity and lipogenic index tended to be lower excluding increased fatty acid synthesis. A selective increase of monounsaturated phosphatidylcholine and phosphatidylethanolamine with the latter showing a positive correlation with systemic triglycerides, which are elevated in APN-/- mice, was identified. SCD1 synthesizes monounsaturated fatty acids and despite higher mRNA expression enzyme activity was not enhanced. Glucosylceramide postulated to contribute to liver damage was decreased. This study demonstrates that adiponectin deficiency is associated with hepatic changes in major lipid classes in mice fed a standard chow which may protect the mice from liver steatosis. |
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Authors:
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Josef Wanninger; Gerhard Liebisch; Gerd Schmitz; Sabrina Bauer; Kristina Eisinger; Markus Neumeier; Andreas Schäffler; Noriyuki Ouchi; Kenneth Walsh; Christa Buechler |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-3-23 |
Journal Detail:
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Title: Experimental and molecular pathology Volume: - ISSN: 1096-0945 ISO Abbreviation: - Publication Date: 2012 Mar |
Date Detail:
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Created Date: 2012-4-2 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370711 Medline TA: Exp Mol Pathol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012. Published by Elsevier Inc. |
Affiliation:
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Department of Internal Medicine I, Regensburg University Hospital, Regensburg, Germany. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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