Document Detail

Lipidomic analysis of the liver identifies changes of major and minor lipid species in adiponectin deficient mice.
MedLine Citation:
PMID:  22465357     Owner:  NLM     Status:  MEDLINE    
Adiponectin protects from hepatic fat storage but adiponectin deficient mice (APN-/-) fed a standard chow do not develop liver steatosis. This indicates that other pathways might be activated to compensate for adiponectin deficiency. An unbiased and comprehensive screen was performed to identify hepatic alterations of lipid classes in these mice. APN-/- mice had decreased hepatic cholesteryl esters while active SREBP2 and systemic total cholesterol were not altered. Upregulation of cytochromes for bile acid synthesis suggests enhanced biliary cholesterol excretion. Analysis of 37 individual fatty acid species showed reduced stearate whereas total fatty acids were not altered. Total amount of triglycerides and phospholipids were equally abundant. A selective increase of monounsaturated phosphatidylcholine and phosphatidylethanolamine which positively correlate with hepatic and systemic triglycerides with the latter being elevated in APN-/- mice, was identified. Stearoyl-CoA desaturase 1 (SCD1) is involved in the synthesis of monounsaturated fatty acids and despite higher mRNA expression enzyme activity was not enhanced. Glucosylceramide postulated to contribute to liver damage was decreased. This study demonstrates that adiponectin deficiency is associated with hepatic changes in lipid classes in mice fed a standard chow which may protect from liver steatosis.
Josef Wanninger; Gerhard Liebisch; Gerd Schmitz; Sabrina Bauer; Kristina Eisinger; Markus Neumeier; Noriyuki Ouchi; Kenneth Walsh; Christa Buechler
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Publication Detail:
Type:  Journal Article     Date:  2012-03-23
Journal Detail:
Title:  Experimental and molecular pathology     Volume:  94     ISSN:  1096-0945     ISO Abbreviation:  Exp. Mol. Pathol.     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-03-18     Completed Date:  2013-05-16     Revised Date:  2014-02-03    
Medline Journal Info:
Nlm Unique ID:  0370711     Medline TA:  Exp Mol Pathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  412-7     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Inc. All rights reserved.
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MeSH Terms
Adiponectin / genetics,  metabolism*
Bile Acids and Salts / metabolism
Cholesterol / metabolism
Cholesterol Esters / metabolism
Cytochromes / metabolism
Dietary Fats / administration & dosage
Fatty Acids / metabolism
Fatty Liver / metabolism
Glucosylceramides / metabolism
Lipid Metabolism*
Liver / metabolism*
Mice, Knockout
Phosphatidylcholines / metabolism
Phosphatidylethanolamines / metabolism
Phospholipids / metabolism
Stearoyl-CoA Desaturase / genetics,  metabolism
Sterol Regulatory Element Binding Protein 2 / metabolism
Triglycerides / metabolism
Grant Support
Reg. No./Substance:
0/Adiponectin; 0/Bile Acids and Salts; 0/Cholesterol Esters; 0/Cytochromes; 0/Dietary Fats; 0/Fatty Acids; 0/Glucosylceramides; 0/Phosphatidylcholines; 0/Phosphatidylethanolamines; 0/Phospholipids; 0/Srebf2 protein, mouse; 0/Sterol Regulatory Element Binding Protein 2; 0/Triglycerides; 39382-08-6/phosphatidylethanolamine; 97C5T2UQ7J/Cholesterol; EC protein, mouse; EC Desaturase

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