| Lipidomic approaches to the study of phospholipase A2-regulated phospholipid fatty acid incorporation and remodeling. | |
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MedLine Citation:
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PMID: 19969040 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The distribution of fatty acids among cellular glycerophospholipids is finely regulated by the CoA-dependent acylation of lysophospholipids followed by transacylation reactions. Arachidonic acid is the fatty acid precursor of a wide family of bioactive compounds called the eicosanoids, with key roles in innate immunity and inflammation. Because availability of free AA constitutes a rate-limiting step in the generation of eicosanoids by mammalian cells, many studies have been devoted to characterize the processes of arachidonate liberation from phospholipids by phospholipase A(2)s and its re-incorporation and further remodeling back into phospholipids by acyltransferases and transacylases. These studies have traditionally been conducted by using radioactive precursors which do not allow the identification of the phospholipid molecular species involved in these processes. Nowadays, lipidomic approaches utilizing mass spectrometry provide a new frame for the analysis of unique phospholipid species involved in fatty acid release and phospholipid incorporation and remodeling. This review focuses on the mass spectrometry techniques applied to the study of phospholipid fatty acid trafficking and the recent advances that have been achieved by the use of this technique. |
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Authors:
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David Balgoma; Olimpio Montero; María A Balboa; Jesús Balsinde |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Review Date: 2009-12-05 |
Journal Detail:
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Title: Biochimie Volume: 92 ISSN: 1638-6183 ISO Abbreviation: Biochimie Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-05-31 Completed Date: 2010-09-02 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 1264604 Medline TA: Biochimie Country: France |
Other Details:
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Languages: eng Pagination: 645-50 Citation Subset: IM |
Copyright Information:
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Copyright 2009 Elsevier Masson SAS. All rights reserved. |
Affiliation:
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Instituto de Biología y Genética Molecular, Consejo Superior de Investigaciones Científicas, 47003 Valladolid, Spain. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arachidonic Acid / metabolism* Fatty Acids / chemistry, metabolism Humans Lipid Metabolism Mass Spectrometry Phospholipases A2 / genetics, metabolism* Phospholipids / chemistry, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Phospholipids; 506-32-1/Arachidonic Acid; EC 3.1.1.4/Phospholipases A2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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