Document Detail

Lipidome profiling of Saccharomyces cerevisiae reveals pitching rate-dependent fermentative performance.
MedLine Citation:
PMID:  20445974     Owner:  NLM     Status:  MEDLINE    
A high cell density strategy has been used in bioethanol production to shorten the fermentation period. To reveal the molecular basis of fermentative behavior in high cell density, the profiling of the phospholipids and sterols of Saccharomyces cerevisiae during fermentation at five different pitching rates (1, 5, 10, 20, and 40 g/L) was investigated. Using LC/ESI/MS(n) technology, 148 phospholipid species were detected, of which 91 species were quantified, and using the gas chromatography-time-of-flight mass spectrometry procedure, a total of 11 sterols were quantified. Phospholipid samples from different pitching rates were discriminated into three groups using principal component analysis (1, 5 g/L, and the others). The main changes in the lipid profile of yeast cells with higher pitching rates were as follows: (a) the relative contents of phosphatidylglycerol and phosphatidylserine were higher while phosphatidylinositol was lower compared with lower pitching rates, (b) the saturated and the relatively shorter fatty acyl chains of phospholipids decreased, and (c) the content of ergosterol was higher. These findings suggested a regulation of the property of the membrane at the situation of high cell density and a possible approach of self-protection of the yeast cells against the high density stresses.
Hong-Chi Tian; Jian Zhou; Bin Qiao; Ying Liu; Jin-Mei Xia; Ying-Jin Yuan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-05-06
Journal Detail:
Title:  Applied microbiology and biotechnology     Volume:  87     ISSN:  1432-0614     ISO Abbreviation:  Appl. Microbiol. Biotechnol.     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-06-28     Completed Date:  2010-09-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8406612     Medline TA:  Appl Microbiol Biotechnol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1507-16     Citation Subset:  IM    
Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University, Tianjin 300072, People's Republic of China.
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MeSH Terms
Phospholipids / chemistry,  metabolism*
Saccharomyces cerevisiae / chemistry*,  metabolism*
Sterols / chemistry,  metabolism*
Reg. No./Substance:
0/Phospholipids; 0/Sterols

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