| Lipid of white-spot syndrome virus originating from host-cell nuclei. | |
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MedLine Citation:
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PMID: 18931090 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The hypothesis that white-spot syndrome virus (WSSV) generates its envelope in the nucleoplasm is based on electron microscopy observations; however, as yet there is no direct evidence for this. In the present study, the lipids of WSSV and the nuclei of its host, the crayfish Procambarus clarkii, were extracted and the neutral lipid and phospholipid contents were analysed by high-performance liquid chromatography, thin-layer chromatography and gas chromatography/mass spectrometry. Phosphatidylcholine (PC) and phosphatidylethanolamine comprised 62.9 and 25.8 %, respectively, of WSSV phospholipids, whereas they comprised 58.5 and 30 %, respectively, of crayfish nuclei phospholipids. These two phospholipids were the dominant phospholipids, and amounts of other phospholipids were very low in the total WSSV and crayfish nuclei phospholipids. The data indicate that the phospholipid profile of WSSV and crayfish nuclei are similar, which is in agreement with the model that the lipids of WSSV are from the host-cell nuclei. However, the fatty acid chains of PC were different between the WSSV virions and crayfish nuclei, and the viral neutral lipid component was also found to be somewhat more complicated than that of the host nuclei. The number of species of cholesterol and hydrocarbon in virus neutral lipid was increased compared with that in host-cell nuclei neutral lipid. It is suggested that the differences between WSSV and its host are either due to selective sequestration of lipids or reflect the fact that the lipid metabolism of the host is changed by WSSV infection. |
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Authors:
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Qing Zhou; Hui Li; Yi-Peng Qi; Feng Yang |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of general virology Volume: 89 ISSN: 0022-1317 ISO Abbreviation: J. Gen. Virol. Publication Date: 2008 Nov |
Date Detail:
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Created Date: 2008-10-20 Completed Date: 2009-01-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0077340 Medline TA: J Gen Virol Country: England |
Other Details:
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Languages: eng Pagination: 2909-14 Citation Subset: IM |
Affiliation:
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Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, SOA, Xiamen, PR China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Astacoidea / virology* Chromatography, High Pressure Liquid Chromatography, Thin Layer Gas Chromatography-Mass Spectrometry Lipids / analysis* Signal Transduction Virion / isolation & purification White spot syndrome virus 1 / chemistry*, isolation & purification, pathogenicity, physiology* |
| Chemical | |
Reg. No./Substance:
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0/Lipids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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