| Lipid remodeling of murine epididymosomes and spermatozoa during epididymal maturation. | |
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MedLine Citation:
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PMID: 16510839 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have isolated vesicular structures from mouse epididymal fluid, referred to as epididymosomes. Epididymosomes have a roughly spherical aspect and a bilayer membrane, and they are heterogeneous in size and content. They originate from the epididymal epithelium, notably from the caput region, and are emitted in the epididymal lumen by way of apocrine secretion. We characterized their membranous lipid profiles in caput and cauda epididymidal fluid samples and found that epididymosomes were particularly rich in sphingomyelin (SM) and arachidonic acid. The proportion of SM increased markedly during epididymal transit and represented half the total phospholipids in cauda epididymidal epididymosomes. The cholesterol:phospholipid ratio increased from 0.26 in the caput to 0.48 in the cauda epididymidis. Measures of epididymosomal membrane anisotropy revealed that epididymosomes became more rigid during epididymal transit, in agreement with their lipid composition. In addition, we have characterized the membrane lipid pattern of murine epididymal spermatozoa during their maturation. Here, we have shown that mouse epididymal spermatozoa were distinguished by high percentages of SM and polyunsaturated membranous fatty acids (PUFAs), principally represented by arachidonic, docosapentanoic, and docosahexanoic acids. Both SM and PUFA increased throughout the epididymal tract. In particular, we observed a threefold rise in the ratio of docosapentanoic acid. Epididymal spermatozoa had a constant cholesterol:phospholipid ratio (average, 0.30) during epididymal transit. These data suggest that in contrast with epididymosomes, spermatozoal membranes seem to become more fluid during epididymal maturation. |
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Authors:
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Hanae Rejraji; Benoit Sion; Gerard Prensier; Martine Carreras; Claude Motta; Jean-Marie Frenoux; Evelyne Vericel; Genevieve Grizard; Patrick Vernet; Joël R Drevet |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-03-01 |
Journal Detail:
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Title: Biology of reproduction Volume: 74 ISSN: 0006-3363 ISO Abbreviation: Biol. Reprod. Publication Date: 2006 Jun |
Date Detail:
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Created Date: 2006-05-22 Completed Date: 2006-09-13 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0207224 Medline TA: Biol Reprod Country: United States |
Other Details:
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Languages: eng Pagination: 1104-13 Citation Subset: IM |
Affiliation:
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Laboratoire Epididyme et Maturation des Gamètes, Université Blaise Pascal, CNRS UMR 6547 GEEM, 63177 Aubière, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anisotropy Arachidonic Acid / analysis Cholesterol / analysis Epididymis / growth & development*, physiology, ultrastructure Extracellular Fluid Fatty Acids / analysis, chemistry Fatty Acids, Unsaturated / analysis Male Membrane Lipids / analysis*, chemistry Mice Organelles / chemistry*, physiology, ultrastructure Phospholipids / analysis Secretory Vesicles / chemistry*, physiology, ultrastructure Sexual Maturation / physiology* Sperm Maturation / physiology Spermatozoa / chemistry*, cytology, physiology, ultrastructure Sphingomyelins / analysis |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Fatty Acids, Unsaturated; 0/Membrane Lipids; 0/Phospholipids; 0/Sphingomyelins; 25448-00-4/docosapentaenoic acid; 506-32-1/Arachidonic Acid; 57-88-5/Cholesterol |
| Comments/Corrections | |
Erratum In:
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Biol Reprod. 2006 Aug;75(2):306 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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