| Lipid profiling by electrospray ionization tandem mass spectrometry and the identification of lipid phosphorylation by kinases in potato stolons. | |
| | |
MedLine Citation:
|
PMID: 22142228 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
There is limited information about the involvement of lipids and esterified fatty acids in signaling pathways during plant development. The purpose of this study was to evaluate the lipid composition and molecular species of potato (Solanum tuberosum L., cv. Spunta) stolons and to identify phosphorylated lipids in the first two developmental stages of tuber formation. Lipid profiling was determined using ESI-MS/MS, a useful method for the determination of the biosynthesis and catabolism of lipids based on their fatty acid composition. The most prevalent compound identified in this study was phosphatidic acid (PA); digalactosyldiacylglycerol (DGDG) was the second most abundant compound. A 34:2 species was identified in PA, phosphatidylcholine (PC), phosphatidylinositol (PI), and phosphatidylethanolamine (PE). The identification of lipid phosphorylation by kinases was revealed by the presence of the phosphorylated lipids. PA was metabolized to diacylglycerol pyrophosphate (DGPP) by phosphatidic acid kinase (PAK). This work establishes a correlation between lipid fatty acid composition and lipid metabolism enzymes at the beginning of tuber formation and is the first report of PAK activity in the early events of potato tuber formation. |
| | |
Authors:
|
Ana M Cenzano; Renata Cantoro; S M Teresa Hernandez-Sotomayor; Guillermina I Abdala; Graciela E Racagni |
Related Documents
:
|
22516248 - Metabolic profiling of a rhizopus oryzae fumaric acid production mutant generated by fe... 869528 - Muramic acid as a measure of microbial biomass in estuarine and marine samples. 21658448 - Investigating the barrier function of skin lipid models with varying compositions. 14740188 - Cerebrosides of candida lipolytica yeast. 110768 - Genetic exchange mechanisms in neisseria gonorrhoeae. 7346268 - Pathways of palmitate metabolism in the isolated rat lung. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-12-5 |
Journal Detail:
|
Title: Journal of agricultural and food chemistry Volume: - ISSN: 1520-5118 ISO Abbreviation: - Publication Date: 2011 Dec |
Date Detail:
|
Created Date: 2011-12-6 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Infliximab salvage therapy after failure of ciclosporin in corticosteroid-refractory ulcerative coli...
Next Document: A General Approach to Low Molecular Weight Metallogelators via Coordination-Induced Gelation of an L...