| Lipid metabolism in arbuscular mycorrhizal roots of Medicago truncatula. | |
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MedLine Citation:
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PMID: 15797604 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The peroxidation of polyunsaturated fatty acids, common to all eukaryotes, is mostly catalyzed by members of the lipoxygenase enzyme family of non-heme iron containing dioxygenases. Lipoxygenase products can be metabolized further in the oxylipin pathway by several groups of CYP74 enzymes. One prominent oxylipin is jasmonic acid (JA), a product of the 13-allene oxide synthase branch of the pathway and known as signaling substance that plays a role in vegetative and propagative plant development as well as in plant responses to wounding and pathogen attack. In barley roots, JA level increases upon colonization by arbuscular mycorrhizal fungi. Apart from this first result regarding JA, no information is available on the relevance of lipidperoxide metabolism in arbuscular mycorrhizal symbiosis. Thus we analyzed fatty acid and lipidperoxide patterns in roots of Medicago truncatula during mycorrhizal colonization. Levels of fungus-specific fatty acids as well as palmitic acid (16:0) and oleic acid (18:1 n - 9) were increased in mycorrhizal roots. Thus the degree of arbuscular mycorrhizal colonization of roots can be estimated via analysis of fungal specific esterified fatty acids. Otherwise, no significant changes were found in the profiles of esterified and free fatty acids. The 9- and 13-LOX products of linoleic and alpha-linolenic acid were present in all root samples, but did not show significant differences between mycorrhizal and non-mycorrhizal roots, except JA which showed elevated levels in mycorrhizal roots. In both types of roots levels of 13-LOX products were higher than those of 9-LOX products. In addition, three cDNAs encoding CYP74 enzymes, two 9/13-hydroperoxide lyases and a 13-allene oxide synthase, were isolated and characterized. The transcript accumulation of these three genes, however, was not increased in mycorrhizal roots of M. truncatula. |
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Authors:
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Michael Stumpe; Jan-Gerrit Carsjens; Irene Stenzel; Cornelia Göbel; Imke Lang; Katharina Pawlowski; Bettina Hause; Ivo Feussner |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Phytochemistry Volume: 66 ISSN: 0031-9422 ISO Abbreviation: Phytochemistry Publication Date: 2005 Apr |
Date Detail:
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Created Date: 2005-03-30 Completed Date: 2005-07-05 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0151434 Medline TA: Phytochemistry Country: United States |
Other Details:
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Languages: eng Pagination: 781-91 Citation Subset: IM |
Affiliation:
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Albrecht-von-Haller Institute of Plant Sciences, Department for Plant Biochemistry, Georg-August-University Göttingen, Justus-von-Liebig-Weg 11, D-37077 Göttingen, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Gene Expression Regulation, Plant
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physiology Lipid Metabolism* Lipid Peroxidation / physiology Lipids / chemistry Medicago truncatula / metabolism*, microbiology* Molecular Structure Mycorrhizae / metabolism, physiology* Plant Proteins / metabolism Plant Roots / metabolism, microbiology Symbiosis / physiology Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Lipids; 0/Plant Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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