| Lipid mapping of colonic mucosa by cluster TOF-SIMS imaging and multivariate analysis in cftr knockout mice. | |
| | |
MedLine Citation:
|
PMID: 20616379 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The cftr knockout mouse model of cystic fibrosis (CF) shows intestinal obstruction; malabsorption and inflammation; and a fatty acid imbalance in intestinal mucosa. We performed a lipid mapping of colon sections from CF and control (WT) mice by cluster time of flight secondary-ion mass spectrometry (TOF-SIMS) imaging to localize lipid alterations. Data were processed either manually or by multivariate statistical methods. TOF-SIMS analysis showed a particular localization for cholesteryl sulfate at the epithelial border, C16:1 fatty acid in Lieberkühn glands, and C18:0 fatty acid in lamina propria and submucosa. Significant increases in vitamin E (vE) and C16:0 fatty acid in the epithelial border of CF colon were detected. Principal component analysis (PCA) and partitioning clustering allowed us to characterize different structural regions of colonic mucosa according to variations in C14:0, C16:0, C16:1, C18:0, C18:1, C18:2, C20:3, C20:4, and C22:6 fatty acids; phosphatidylethanolamine, phosphatidylcholine, and phosphatidylinositol glycerolipids; cholesterol; vitamin E; and cholesteryl sulfate. PCA on spectra from Lieberkühn glands led to separation of CF and WT individuals. This study shows for the first time the spatial distribution of lipids in colonic mucosa and suggests TOF-SIMS plus multivariate analyses as a powerful tool to investigate disease-related tissue spatial lipid signatures. |
| | |
Authors:
|
Marc Brulet; Alexandre Seyer; Aleksander Edelman; Alain Brunelle; Janine Fritsch; Mario Ollero; Olivier Laprévote |
Related Documents
:
|
1401029 - Identification of some uncommon monounsaturated fatty acids of bacteria. 16348709 - Effect of growth temperature on fatty acid composition of ten thermus strains. 3183519 - Fatty acids of pulmonary surfactant phosphatidylcholine from fetal rabbit lung tissue i... 6284229 - Reduction of opioid binding in neuroblastoma x glioma cells grown in medium containing ... 3178759 - Species pattern of phosphatidylinositol from lung surfactant and a comparison of the sp... 7103169 - Fatty acid composition of equine erythrocytes. 786079 - Mutagenicity of volatile anesthetics: halothane. 2984699 - Pepsinogen synthesis and secretion by isolated gastric glands. 21281409 - Biotechnological conversion of waste cooking olive oil into lipid-rich biomass using as... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-07-08 |
Journal Detail:
|
Title: Journal of lipid research Volume: 51 ISSN: 0022-2275 ISO Abbreviation: J. Lipid Res. Publication Date: 2010 Oct |
Date Detail:
|
Created Date: 2010-09-10 Completed Date: 2011-01-24 Revised Date: 2012-04-27 |
Medline Journal Info:
|
Nlm Unique ID: 0376606 Medline TA: J Lipid Res Country: United States |
Other Details:
|
Languages: eng Pagination: 3034-45 Citation Subset: IM |
Affiliation:
|
Centre de Recherche de Gif, Institut de Chimie des Substances Naturelles, CNRS, Gif-sur-Yvette, France. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Colon / chemistry*, metabolism Cystic Fibrosis Transmembrane Conductance Regulator / genetics*, metabolism Intestinal Mucosa / chemistry* Lipid Metabolism Lipids / analysis* Mice Mice, Knockout Multivariate Analysis Principal Component Analysis Spectrometry, Mass, Secondary Ion / methods* |
| Chemical | |
Reg. No./Substance:
|
0/Lipids; 126880-72-6/Cystic Fibrosis Transmembrane Conductance Regulator |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Acute ANIT toxicity in male IL-10 knockout and wild-type mice.
Next Document: Putative mutation mechanism and light responses of a protochlorophyllide oxidoreductase-less barley ...