Document Detail


Lipid and lipid mediator profiling of human synovial fluid in rheumatoid arthritis patients by means of LC-MS/MS.
MedLine Citation:
PMID:  22841830     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human synovial fluid (SF) provides nutrition and lubrication to the articular cartilage. Particularly in arthritic diseases, SF is extensively accumulating in the synovial junction. During the last decade lipids have attracted considerable attention as their role in the development and resolution of diseases became increasingly recognized. Here, we describe a capillary LC-MS/MS screening platform that was used for the untargeted screening of lipids present in human SF of rheumatoid arthritis (RA) patients. Using this platform we give a detailed overview of the lipids and lipid-derived mediators present in the SF of RA patients. Almost 70 different lipid components from distinct lipid classes were identified and quantification was achieved for the lysophosphatidylcholine and phosphatidylcholine species. In addition, we describe a targeted LC-MS/MS lipid mediator metabolomics strategy for the detection, identification and quantification of maresin 1, lipoxin A(4) and resolvin D5 in SF from RA patients. Additionally, we present the identification of 5S,12S-diHETE as a major marker of lipoxygenase pathway interactions in the investigated SF samples. These results are the first to provide a comprehensive approach to the identification and profiling of lipids and lipid mediators present in SF and to describe the presence of key anti-inflammatory and pro-resolving lipid mediators identified in SF from RA patients.
Authors:
Martin Giera; Andreea Ioan-Facsinay; Rene Toes; Fei Gao; Jesmond Dalli; André M Deelder; Charles N Serhan; Oleg A Mayboroda
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-07-24
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1821     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-09-04     Completed Date:  2012-11-08     Revised Date:  2013-11-06    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1415-24     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier B.V. All rights reserved.
Affiliation:
Biomolecular Mass Spectrometry Unit, Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands. m.a.giera@lumc.nl
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MeSH Terms
Descriptor/Qualifier:
Arthritis, Rheumatoid / diagnosis,  metabolism*
Biological Markers / metabolism*
Chromatography, Liquid
Humans
Lipid Metabolism
Lipids / analysis*
Metabolomics*
Synovial Fluid / metabolism*
Tandem Mass Spectrometry
Grant Support
ID/Acronym/Agency:
1P01GM095467/GM/NIGMS NIH HHS; P01 GM095467/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Lipids
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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