Document Detail

Lipid A in Helicobacter pylori.
MedLine Citation:
PMID:  1398948     Owner:  NLM     Status:  MEDLINE    
Free lipid A of Helicobacter pylori was characterized with regard to chemical composition, reactivity with anti-lipid A antibodies, and activity in a Limulus lysate assay. The predominant fatty acids of H. pylori lipid A were 3-OH-18:0, 18:0, 3-OH-16:0, 16:0, and 14:0. Hexosamine was present in amounts similar to those in Campylobacter jejuni or Salmonella typhimurium lipid A. The lipopolysaccharide of H. pylori contained 2-keto-3-deoxyoctonic acid, a common constituent of enterobacterial and C. jejuni lipopolysaccharides. In the enzyme-linked immunosorbent assay, the doses of lipid A required to inhibit anti-lipid A by 50% (EI50 values) by absorption of the immune (rabbit) serum were 7.9, 1.2, and 1.4 micrograms of O-deacylated lipid A's from H. pylori, C. jejuni, and S. typhimurium per ml, respectively. The lower reactivity of H. pylori lipid A compared with those of the other two lipid A preparations (as shown by the higher EI50 value) was underscored by the use of a murine monoclonal anti-lipid A antibody in the inhibition assay. An EI50 value was not obtained at the concentrations tested for H. pylori lipid A; the corresponding figures for C. jejuni and S. typhimurium lipid A's were 13 and 14 micrograms/ml, respectively. No inhibition was obtained with H. pylori lipopolysaccharide, which showed a low-molecular-weight profile on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The activity of H. pylori lipid A in the Limulus assay was approximately 71 and 650 times lower than those of C. jejuni and S. typhimurium lipid A's, respectively. These findings suggest that lipid A is an integral part of the outer cell wall of H. pylori. The lower reactivity of H. pylori lipid A with anti-lipid A antibodies and in the Limulus assay compared with that of C. jejuni or S. typhimurium lipid A may be explained by a different composition of the fatty acids, especially the 3-hydroxy fatty acids, and a possible deviating phosphorylation pattern.
I Mattsby-Baltzer; Z Mielniczuk; L Larsson; K Lindgren; S Goodwin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Infection and immunity     Volume:  60     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  1992 Oct 
Date Detail:
Created Date:  1992-10-26     Completed Date:  1992-10-26     Revised Date:  2010-09-07    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4383-7     Citation Subset:  IM    
Department of Clinical Bacteriology, University of Göteborg, Sweden.
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MeSH Terms
Antibodies, Monoclonal / immunology
Helicobacter pylori / chemistry*,  pathogenicity
Limulus Test
Lipid A / analysis*,  immunology,  toxicity
Lipopolysaccharides / analysis,  immunology
Mice, Inbred BALB C
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Lipid A; 0/Lipopolysaccharides

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