Document Detail

Lipid hydroperoxide measurement by oxidation of Fe2+ in the presence of xylenol orange. Comparison with the TBA assay and an iodometric method.
MedLine Citation:
PMID:  1795606     Owner:  NLM     Status:  MEDLINE    
Study of the role of hydroperoxides and lipid peroxidation in disease requires simple and sensitive methods for direct hydroperoxide measurement. We report on a technique for measuring hydroperoxide which relies upon the rapid hydroperoxide-mediated oxidation of Fe2+ under acidic conditions. Fe3+ forms a chromophore with xylenol orange which absorbs strongly at 560 nm, yielding an apparent E560 (for H2O2, butyl hydroperoxide and cumene hydroperoxide) of 4.3 X 10(4) M-1 cm-1. The assay was validated in a study of liposomal lipid peroxidation and shown to give results comparable with those obtained by an iodometric method or by measuring conjugated dienes. The assay involving thiobarbituric acid, by comparison, underestimates lipid peroxidation and does not measure hydroperoxide per se.
Z Y Jiang; A C Woollard; S P Wolff
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Lipids     Volume:  26     ISSN:  0024-4201     ISO Abbreviation:  Lipids     Publication Date:  1991 Oct 
Date Detail:
Created Date:  1992-04-09     Completed Date:  1992-04-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0060450     Medline TA:  Lipids     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  853-6     Citation Subset:  IM    
Department of Clinical Pharmacology, University College and Middlesex School of Medicine, London, United Kingdom.
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MeSH Terms
Enzymes / metabolism
Ferrous Compounds
Lipid Peroxides / analysis*,  metabolism
Liposomes / analysis*
Reg. No./Substance:
0/Enzymes; 0/Ferrous Compounds; 0/Lipid Peroxides; 0/Liposomes; 0/Oxidants; 0/Thiobarbiturates; 0/Xylenes; 1611-35-4/xylenol orange; 504-17-6/thiobarbituric acid; 7440-50-8/Copper; 7553-56-2/Iodine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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