| Lipid and fatty acid analysis of fresh and frozen-thawed immature and in vitro matured bovine oocytes. | |
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MedLine Citation:
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PMID: 11425337 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The lipid content and fatty acid composition of fresh immature and in vitro matured bovine oocytes cultured in media with or without serum, and also those of frozen-thawed immature oocytes were analysed. All oocytes were ranked (A or B) on the basis of their cytoplasmic quality. Fatty acid composition (mol %; w/w) in the total lipid fraction was analysed by gas chromatography. Triglyceride, total cholesterol, phospholipid (phosphocholine-containing phospholipid) and non-esterified fatty acid contents of immature and in vitro matured oocytes were determined using lipid analysis kits. Phosphocholine-containing phospholipid and non-esterified fatty acid contents were determined in frozen-thawed immature bovine oocytes. Palmitic acid was the most abundant fatty acid in immature oocytes (A: 35%, B: 36%), and in in vitro matured oocytes cultured in the medium containing serum (A: 36%, B: 35%) or polyvinyl alcohol (A: 33%, B: 36%). Oleic acid was the second most abundant fatty acid in all A ranked oocytes, whereas stearic acid was the second most abundant fatty acid in all B ranked oocytes. There were significant differences (P < 0.05) in linoleic and arachidonic acid fractions between A and B ranked immature oocytes. In vitro matured oocytes had significantly (P < 0.05) lower proportions of linoleic and arachidonic acids, and significantly (P < 0.01) lower contents of triglyceride and total cholesterol compared with those of immature oocytes. The fatty acid composition of in vitro matured oocytes cultured in medium containing fetal calf serum or polyvinyl alcohol was similar, but significant differences (P < 0.01) in triglyceride and the total cholesterol content were observed. There was a significant decrease (P < 0.05) in the arachidonic acid proportion in frozen-thawed immature oocytes compared with that in fresh immature oocytes. In addition, significant (P < 0.05) decreases in both phospholipid (15.8--10.6 pmol) and non-esterified fatty acid (11.0--4.1 pmol) were found in frozen--thawed immature oocytes. The results indicate that lipids are available for use as an energy source for maturation and that serum lipids are incorporated into the oocyte cytoplasm during in vitro maturation. The changes in the lipid content (mainly phospholipid) and fatty acid composition were also observed in frozen--thawed immature oocytes. The study indicates that the alteration of fatty acid composition in bovine oocytes might improve maturation and cryopreservation. |
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Authors:
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J Y Kim; M Kinoshita; M Ohnishi; Y Fukui |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Reproduction (Cambridge, England) Volume: 122 ISSN: 1470-1626 ISO Abbreviation: Reproduction Publication Date: 2001 Jul |
Date Detail:
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Created Date: 2001-06-26 Completed Date: 2001-10-11 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 100966036 Medline TA: Reproduction Country: England |
Other Details:
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Languages: eng Pagination: 131-8 Citation Subset: IM |
Affiliation:
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Laboratory of Animal Genetics and Reproduction, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arachidonic Acid / analysis Cattle Cells, Cultured Cholesterol / analysis Cryopreservation* Culture Media Fatty Acids / analysis* Fatty Acids, Nonesterified / analysis Female Hot Temperature Linoleic Acid / analysis Lipids / analysis* Oleic Acid / analysis Oocytes / chemistry*, physiology* Phospholipids / analysis Phosphorylcholine / analysis Polyvinyl Alcohol Stearic Acids / analysis Triglycerides / analysis |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/Fatty Acids; 0/Fatty Acids, Nonesterified; 0/Lipids; 0/Phospholipids; 0/Stearic Acids; 0/Triglycerides; 107-73-3/Phosphorylcholine; 112-80-1/Oleic Acid; 2197-37-7/Linoleic Acid; 506-32-1/Arachidonic Acid; 57-11-4/stearic acid; 57-88-5/Cholesterol; 9002-89-5/Polyvinyl Alcohol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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