Document Detail

Lipid content and fatty acid composition of green algae Scenedesmus obliquus grown in a constant cell density apparatus.
MedLine Citation:
PMID:  11539709     Owner:  NASA     Status:  MEDLINE    
The lipids of alga Scenedesmus obliquus grown under controlled conditions were separated and fractionated by column and thin-layer chromatography, and fatty acid composition of each lipid component was studied by gas-liquid chromatography (GLC). Total lipids were 11.17%, and neutral lipid, glycolipid and phospholipid fractions were 7.24%, 2.45% and 1.48% on a dry weight basis, respectively. The major neutral lipids were diglycerides, triglycerides, free sterols, hydrocarbons and sterol esters. The glycolipids were: monogalactosyl diglyceride, digalactosyl diglyceride, esterified sterol glycoside, and sterol glycoside. The phospholipids included: phosphatidyl choline, phosphatidyl glycerol and phosphatidyl ethanolamine. Fourteen fatty acids were identified in the four lipid fractions by GLC. The main fatty acids were C18:2, C16:0, C18:3(alpha), C18:1, C16:3, C16:1, and C16:4. Total unsaturated fatty acid and essential fatty acid compositions of the total algal lipids were 80% and 38%, respectively.
K J Choi; Z Nakhost; E Barzana; M Karel
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Food biotechnology     Volume:  1     ISSN:  0890-5436     ISO Abbreviation:  Food Biotechnol     Publication Date:  1987  
Date Detail:
Created Date:  1997-10-10     Completed Date:  1997-10-10     Revised Date:  2007-08-01    
Medline Journal Info:
Nlm Unique ID:  8802223     Medline TA:  Food Biotechnol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  117-28     Citation Subset:  S    
Department of Applied Biological Sciences, Massachusetts Institute of Technology, Cambridge 02139, USA.
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MeSH Terms
Algae, Green / chemistry*,  growth & development
Chromatography, Gas / methods
Chromatography, Thin Layer / methods
Environment, Controlled
Fatty Acids / analysis*,  isolation & purification*
Glycolipids / analysis
Lipids / analysis*,  isolation & purification*
Phospholipids / analysis
Reg. No./Substance:
0/Fatty Acids; 0/Glycolipids; 0/Lipids; 0/Phospholipids
M Karel / MA Inst Tech, Cambridge

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