| Lipid composition and protein profiles of outer and inner membranes from pig heart mitochondria. Comparison with microsomes. | |
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MedLine Citation:
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PMID: 1247555 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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1. Mitochondria, inner and outer mitochondrial membranes and microsomes were isolated and purified from pig heart. Their lipid composition and protein components were studied. 2. The fatty acid distribution in the main phospholipids seemed specific rather of a given phospholipid and not of one type of membrane. 3. Inner mitochondrial membranes were characterized by a high content in cardiolipin and a very low level of triglycerides together with a high degree of unsaturation and C18 acids. Gel electrophoresis revealed 13 different polypeptide subunits of which 5 were major ranging in molecular weights from 10000 to 215000. 4. In outer mitochondrial membranes, total lipid, phosphatidylcholine, phosphatidylinositol, plasmologen and triglyceride contents were much higher than in inner membranes. Fatty acids of phospholipids were mostly saturated and the polypeptide pattern showed 12 components, of which 4 were major of mol. wt 75000, 60000, 20000 and below 10000. 5. Compared to outer membrane, microsomes exhibited a much higher cholesterol content and markedly different protein profiles. They contained significant amounts of cardiolipin and phosphatidylserine, this latter phospholipid being exclusively located in microsomes. However odd similarities were observed in some lipid components of microsomes and inner mitochondrial membranes, but fatty acids were more saturated in microsomes and electrophoretic profiles of protein components appeared very different and revealed components of high mol. wt. |
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Authors:
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J Comte; B Maïsterrena; D C Gautheron |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 419 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1976 Jan |
Date Detail:
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Created Date: 1976-04-09 Completed Date: 1976-04-09 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 271-84 Citation Subset: IM |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Fatty Acids / analysis Glycerides / analysis Lipids / analysis* Membranes / analysis Microsomes / analysis* Mitochondria, Muscle / analysis* Muscle Proteins / analysis* Muscles / analysis* Myocardium Phospholipids / analysis Swine Ubiquinone / analysis |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Glycerides; 0/Lipids; 0/Muscle Proteins; 0/Phospholipids; 1339-63-5/Ubiquinone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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