Document Detail


Lipid composition of axolemma-enriched fractions from human brains.
MedLine Citation:
PMID:  7240954     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The lipid composition was determined for axolemma-enriched fractions and myelin which were isolated via a preparation of purified myelinated axons. The myelin had a lipid composition which was compatible with that previously reported for myelin isolated by alternative procedures. The most dense axolemma-enriched fraction contained 25.3% cholesterol, 25.8% galactolipid (21.3% cerebrosides and 4.8% sulfatides), and 48.9% phospholipid. The major phospholipids were the ethanolamine phospholipid (19.8% of total lipid weight; 49.0% in the plasmalogen form) and choline phospholipids (18.7% of total lipid weight; 16.0% in the plasmalogen form) with lesser amounts of sphingomyelin, phosphatidylserine, and phosphatidylinositol also present; the ganglioside content was 13.9 micrograms of acetylneuraminic acid per mg protein. The less dense axolemma-enriched fraction had a lipid composition which was intermediate between that of myelin and the more dense axolemma-enriched fraction. On the average, less than 2.3% of the total protein in the axolemma-enriched fraction was myelin basic protein. Both axolemma-enriched fractions stained uniformly with Luxol fast blue and demonstrated specific saxitoxin-binding which was enriched 2- to 7-fold over that of the whole white matter homogenate from which the fractions were isolated. The choline and ethanolamine phospholipids in that most dense axolemma-enriched fractions contained a greater percentage of unsaturated fatty acids compared with the comparable phospholipids in myelin. The content of unsaturated fatty acids in these phospholipids of the axolemma-enriched fraction was not as great as that of human CNS synaptic plasma membranes. However, the chain length distribution of these phospholipid fatty acids was similar in myelin, synaptic plasma membrane, and the axolemma-enriched fraction. The distribution of aldehydes derived from the ethanolamine phospholipids of the more dense axolemma-enriched fraction closely resemble the distribution of the comparable aldehydes in the myelin fraction. The possible origin and function of the lipids in the axolemma-enriched fractions are discussed.
Authors:
G H DeVries; W J Zetusky; C Zmachinski; V P Calabrese
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of lipid research     Volume:  22     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  1981 Feb 
Date Detail:
Created Date:  1981-08-10     Completed Date:  1981-08-10     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  208-16     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Aged
Axons / analysis*
Brain Chemistry
Fatty Acids / analysis
Humans
Membrane Lipids / analysis*
Middle Aged
Myelin Sheath / analysis
Phosphatidylcholines / analysis
Phosphatidylethanolamines / analysis
Saxitoxin / metabolism
Subcellular Fractions / metabolism
Grant Support
ID/Acronym/Agency:
NS10821/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Membrane Lipids; 0/Phosphatidylcholines; 0/Phosphatidylethanolamines; 35523-89-8/Saxitoxin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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