| Lipid composition of Spodoptera frugiperda (Sf9) and Trichoplusia ni (Tn) insect cells used for baculovirus infection. | |
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MedLine Citation:
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PMID: 9877163 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The lipid composition of two different insect cell lines from Spodoptera frugiperda (Sf9) and Trichoplusia ni (Tn) which are established cell lines for infection with recombinant baculovirus was analyzed by high-performance liquid chromatography and gas-liquid chromatography. The major phospholipids found were phosphatidylcholine and phosphatidylethanolamine, the major mono-unsaturated fatty acids were oleic acid and palmitoleic acid, the major saturated fatty acid was stearic acid. The cholesterol to phospholipid ratio was demonstrated to be lower than in mammalian cell lines. Infection with a recombinant baculovirus Autographa californica resulted in increased levels of phosphatidylcholine in the insect cells. The baculovirus/insect cell system has become a popular system for heterologous protein production. Functional changes of membrane proteins produced in these two cell lines might be correlated to a different lipid profile of their cellular membranes. |
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Authors:
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K Marheineke; S Grünewald; W Christie; H Reiländer |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: FEBS letters Volume: 441 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 1998 Dec |
Date Detail:
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Created Date: 1999-01-21 Completed Date: 1999-01-21 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 49-52 Citation Subset: IM |
Affiliation:
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Max-Planck-Institut für Biophysik, Abteilung Molekulare Membranbiologie, Frankfurt/M, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Baculoviridae* Cell Line / chemistry Chromatography, Gas Chromatography, High Pressure Liquid Insects / chemistry* Lipids / analysis* Phospholipids / analysis Spodoptera / chemistry* Transfection / methods |
| Chemical | |
Reg. No./Substance:
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0/Lipids; 0/Phospholipids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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