Document Detail


Lipid biosynthesis and its coordination with cell cycle progression.
MedLine Citation:
PMID:  16239308     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The activation of cell cycle regulators at the G1/S boundary has been linked to the cellular protein synthesis rate. It is conceivable that regulatory mechanisms are required to allow cells to coordinate the synthesis of other macromolecules with cell cycle progression. The availability of highly synchronized cells and flow cytometric methods facilitates investigation of the dynamics of lipid synthesis in the entire cell cycle of the heterotrophic dinoflagellate Crypthecodinium cohnii. Flow cytograms of Nile red-stained cells revealed a stepwise increase in the polar lipid content and a continuous increase in neutral lipid content in the dinoflagellate cell cycle. A cell cycle delay at early G1, but not G2/M, was observed upon inhibition of lipid synthesis. However, lipid synthesis continued during cell cycle arrest at the G1/S transition. A cell cycle delay was not observed when inhibitors of cellulose synthesis and fatty acid synthesis were added after the late G1 phase of the cell cycle. This implicates a commitment point that monitors the synthesis of fatty acids at the late G1 phase of the dinoflagellate cell cycle. Reduction of the glucose concentration in the medium down-regulated the G1 cell size with a concomitant forward shift of the commitment point. Inhibition of lipid synthesis up-regulated cellulose synthesis and resulted in an increase in cellulosic contents, while an inhibition of cellulose synthesis had no effects on lipid synthesis. Fatty acid synthesis and cellulose synthesis are apparently coupled to the cell cycle via independent pathways.
Authors:
Alvin C M Kwok; Joseph T Y Wong
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Publication Detail:
Type:  Journal Article     Date:  2005-10-20
Journal Detail:
Title:  Plant & cell physiology     Volume:  46     ISSN:  0032-0781     ISO Abbreviation:  Plant Cell Physiol.     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2005-12-29     Completed Date:  2006-02-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9430925     Medline TA:  Plant Cell Physiol     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1973-86     Citation Subset:  IM    
Affiliation:
Department of Biology, Hong Kong University of Science and Technology, Clearwater Bay, Kowloon, Hong Kong SAR, PR China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Cycle / drug effects,  physiology*
Cell Division / drug effects,  physiology
Cellulose / biosynthesis
Cerulenin / pharmacology
Dinoflagellida / chemistry,  cytology*,  drug effects,  metabolism*
Fatty Acids / biosynthesis
Flow Cytometry
G1 Phase / drug effects,  physiology
G2 Phase / drug effects,  physiology
Glucose / pharmacology
Hydroxyurea / pharmacology
Lipids / biosynthesis*
Nitriles / pharmacology
S Phase / drug effects,  physiology
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Lipids; 0/Nitriles; 1194-65-6/dichlobanil; 127-07-1/Hydroxyurea; 17397-89-6/Cerulenin; 50-99-7/Glucose; 9004-34-6/Cellulose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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