| Lipid biomarker and phylogenetic analyses to reveal archaeal biodiversity and distribution in hypersaline microbial mat and underlying sediment. | |
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MedLine Citation:
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PMID: 18564188 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study has utilized the tools of lipid biomarker chemistry and molecular phylogenetic analyses to assess the archaeal contribution to diversity and abundance within a microbial mat and underlying sediment from a hypersaline lagoon in Baja California. Based on abundance of ether-linked isoprenoids, archaea made up from 1 to 4% of the cell numbers throughout the upper 100 mm of mat and sediment core. Below this depth archaeal lipid was two times more abundant than bacterial. Archaeol was the primary archaeal lipid in all layers. Relatively small amounts of caldarchaeol (dibiphytanyl glyceroltetraether) were present at most depths with phytanyl to biphytanyl molar ratios lowest (approximately 10 : 1) in the 4-17 mm and 100-130 mm horizons, and highest (132 : 1) in the surface 0-2 mm. Lipids with cyclic biphytanyl cores were only detected below 100 mm. A novel polar lipid containing a C(30) isoprenoid (squalane) moiety was isolated from the upper anoxic portion of the core and partially characterized. Hydrocarbon biomarker lipids included pentamethylicosane (2-10 mm) and crocetane (primarily below 10 mm). Archaeal molecular diversity varied somewhat with depth. With the exception of samples at 0-2 mm and 35-65 mm, Thermoplasmatales of marine benthic group D dominated clone libraries. A significant number of phylotypes representing the Crenarchaeota from marine benthic group B were generally present below 17 mm and dominated the 35-65 mm sample. Halobacteriaceae family made up 80% of the clone library of the surface 2 mm, and consisted primarily of sequences affiliated with the haloalkaliphilic Natronomonas pharaonis. |
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Authors:
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L L Jahnke; V J Orphan; T Embaye; K A Turk; M D Kubo; R E Summons; D J DES Marais |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2008-06-28 |
Journal Detail:
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Title: Geobiology Volume: 6 ISSN: 1472-4669 ISO Abbreviation: Geobiology Publication Date: 2008 Aug |
Date Detail:
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Created Date: 2008-09-16 Completed Date: 2008-10-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101185472 Medline TA: Geobiology Country: England |
Other Details:
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Languages: eng Pagination: 394-410 Citation Subset: IM |
Affiliation:
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Space Science Division, NASA Ames Research Center, Moffett Field, CA 94035, USA. linda.l.jahnke@nasa.gov |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/EU570118; EU570119; EU570120; EU570121; EU570122; EU570123; EU570124; EU570125; EU570126; EU570127; EU570128; EU570129; EU570130; EU570131; EU570132; EU570133; EU570134; EU570135; EU570136; EU570137; EU570138; EU570139; EU570140; EU570141; EU570142; EU570143; EU570144; EU570145; EU570146; EU570147; EU570148; EU570149; EU570150; EU570151; EU570152; EU570153; EU570154; EU570155; EU570156; EU570157; EU570158; EU570159 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Archaea
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chemistry,
classification*,
genetics,
isolation & purification* Bacteria / isolation & purification Biodiversity* Biological Markers DNA, Archaeal / chemistry, genetics* DNA, Ribosomal / chemistry, genetics Genes, rRNA Lipids / analysis* Mexico Molecular Sequence Data Phylogeny RNA, Archaeal / genetics RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Sequence Homology, Nucleic Acid Water Microbiology* |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/DNA, Archaeal; 0/DNA, Ribosomal; 0/Lipids; 0/RNA, Archaeal; 0/RNA, Ribosomal, 16S |
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