Document Detail


Lipid analysis of normal dermis and hypertrophic scars.
MedLine Citation:
PMID:  18028131     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Hypertrophic scars (HS) are a consequence of abnormal wound healing. We examined fatty acids that are contained within, and participate in, every reaction through the membrane; then, we analyzed the percentage composition of the fatty acids in deepithelialized normal dermis (ND) and HS. In vivo HS samples were obtained from six patients undergoing surgical excision, and ND samples from five patients undergoing skin grafting surgery for excess. In vitro, cultured fibroblasts from HS and ND were also analyzed. The percentage composition of fatty acids extracted from all the samples was analyzed. In vivo, arachidonic acid (20:4) was significantly more abundant in HS than in ND, in the phospholipids from both whole tissue and cell membranes. In vitro, there were no significant differences among ND, HS, and 10% fetal calf serum. The results suggest that HS formation does not necessarily involve simple excess of 20:4; however, there are considerable differences in the percentage composition of 20:4 between ND and HS. Arachidonic acid probably participates in the formation and maintenance of HS, whereas in vitro cultured fibroblasts are affected largely by fetal calf serum.
Authors:
Tadashi Nomura; Hiroto Terashi; Makoto Omori; Atsushi Sakurai; Takeru Sunagawa; Masumi Hasegawa; Shinya Tahara
Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society     Volume:  15     ISSN:  1067-1927     ISO Abbreviation:  Wound Repair Regen     Publication Date:    2007 Nov-Dec
Date Detail:
Created Date:  2007-11-21     Completed Date:  2008-03-07     Revised Date:  2008-07-17    
Medline Journal Info:
Nlm Unique ID:  9310939     Medline TA:  Wound Repair Regen     Country:  United States    
Other Details:
Languages:  eng     Pagination:  833-7     Citation Subset:  IM    
Affiliation:
Department of Plastic Surgery [corrected], Kobe University Graduate School of Medicine, Kobe, Japan. tadnomura-tky@umin.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Arachidonic Acid / analysis
Cicatrix, Hypertrophic / metabolism*
Dermis / metabolism*
Fatty Acids / analysis*
Fibroblasts / metabolism
Humans
Phospholipids / analysis
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Phospholipids; 506-32-1/Arachidonic Acid
Comments/Corrections
Erratum In:
Wound Repair Regen. 2008 May-Jun;16(3):468

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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