Document Detail


Lipid analysis of Helicobacter pylori.
MedLine Citation:
PMID:  8283008     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lipids from Helicobacter pylori were extracted, isolated by conventional DEAE-Sephadex and silica gel column chromatography, and then purified by preparative thin-layer chromatography. Simple and phospholipids were analyzed by HPTLC and quantitatively determined by densitometry scanning. The fatty acid compositions of simple lipids were estimated by gas-liquid chromatography. The simple lipid composition of H. pylori consisted of wax ester (2.5%), triglycerides (4.9%), free fatty acids (30.0%), cholesterol (6.9%), diacylglycerol (29.1%), and monoacylglycerol (2.6%). The neutral phospholipid composition consisted of phosphatidyl ethanolamine (79.1%), lysophosphatidyl ethanolamine (16%), and phosphatidyl choline (1.9%). That of acidic phospholipids consisted of phosphatidic acid (52.7%) and phosphatidyl serine (47.3%). The major fatty acids of crude lipid, wax, free fatty acids, triglycerides, diacylglycerides, and monoacyl glycerides were C19:1 (cyclo), C18:2, C16:0, C19:1 (cyclo), C22h:0, and C18:0, respectively. These results are new findings and suggest significant characteristics from the metabolic, physiologic, and chemotaxonomic point of view.
Authors:
Y Inamoto; S Ariyama; Y Hamanaka; K Okita; Y Kaneta; T Nagate; I Kondou; T Takemoto
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of clinical gastroenterology     Volume:  17 Suppl 1     ISSN:  0192-0790     ISO Abbreviation:  J. Clin. Gastroenterol.     Publication Date:  1993  
Date Detail:
Created Date:  1994-02-17     Completed Date:  1994-02-17     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7910017     Medline TA:  J Clin Gastroenterol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  S136-9     Citation Subset:  IM    
Affiliation:
Department of Internal Medicine, Yamaguchi-Rosai Hospital, Japan.
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MeSH Terms
Descriptor/Qualifier:
Chromatography, Gas
Chromatography, Thin Layer
Fatty Acids / analysis
Glycolipids / analysis
Helicobacter pylori / chemistry*
Humans
Membrane Lipids / analysis*
Phospholipids / analysis
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Glycolipids; 0/Membrane Lipids; 0/Phospholipids

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