Document Detail


Lipid accumulation and metabolism in polychaete spermatogenesis: Role of the large discoidal lipoprotein.
MedLine Citation:
PMID:  20544837     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In most oviparous animals, lipoprotein-mediated lipid transport plays an important role in the nutrient supply for the oocyte. In male gametes, lipids are used as energy substrates in spermatozoa but nothing is yet known about their origin and metabolism throughout spermatogenesis. The lipid profiles analyzed from different stages of male germ cell development in the marine annelid Nereis virens were found to undergo a dramatic change from primary triacylglycerides at the beginning of germ cell development to cholesterol and phospholipids at the end of development as demonstrated by HPLC with evaporative light scattering detection and mass spectrometry. The uptake of a large discoidal lipoprotein into the developing germ cells could be demonstrated by fluorescence labeling and electron microscopic techniques as well as by the presence of a lipoprotein receptor in the germ cells, thus establishing its role in lipid supply. The incorporated lipoprotein discs were found to be stored as intact complexes indicating that they are not readily degraded upon endocytotic uptake. The change in lipid composition during germ cell development reflects their metabolic activity, especially in spermatogonia. The high concentration of lipids maintained by spermatogonia during the early phase of gametogenesis seems to be required for the later rapid processes of meiosis and spermatocyte differentiation. At times when peak demand of lipids arises for membrane synthesis and increased metabolism, this may be met more efficiently by a rapid on-site mobilization of lipids instead of an external supply.
Authors:
Sven Schenk; Ulrich Hoeger
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular reproduction and development     Volume:  77     ISSN:  1098-2795     ISO Abbreviation:  Mol. Reprod. Dev.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-23     Completed Date:  2010-12-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8903333     Medline TA:  Mol Reprod Dev     Country:  United States    
Other Details:
Languages:  eng     Pagination:  710-9     Citation Subset:  IM    
Copyright Information:
(c) 2010 Wiley-Liss, Inc.
Affiliation:
Institut für Zoologie, Johannes Gutenberg-Universität, Mainz, Germany. schenks@uni-mainz.de
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Transport / physiology
Cells, Cultured
Germ Cells / growth & development,  physiology
Lipid Metabolism / physiology*
Lipoproteins / chemistry,  metabolism,  physiology*
Male
Molecular Weight
Polychaeta / metabolism*,  physiology*
Receptors, Lipoprotein / metabolism
Spermatogenesis / physiology*
Spermatozoa / growth & development,  metabolism,  physiology,  ultrastructure
Chemical
Reg. No./Substance:
0/Lipoproteins; 0/Receptors, Lipoprotein; 0/discoidal lipoproteins

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