| Lipid accumulation cells derived from porcine aorta and grown under anaerobic conditions. | |
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MedLine Citation:
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PMID: 994749 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Fibroblast-like cells, derived from porcine aorta, were cultured under aerobic and anaerobic conditions. Light and electron microscopic examinations, lipid composition measurements, and incorporation of radioactive precursors into lipids of these cells were performed. Anaerobically grown cells accumulated oil red O stainable droplets and within 6 hr the triacylglycerol content increased to 4 times the level determined in cells grown under aerobic conditions. This ratio remained constant throughout an additional 12 hr of growth. The fatty acid composition of the triacylglycerols which accumulated under anaerobic conditions differed from the composition of fatty acids in the triacylglycerols present in the growth medium. The cellular unesterified fatty acids of the anaerobically grown cells differed only slightly in composition from the fatty acids in the growth medium, while the unesterfied fatty acids of aerobically grown cells differed to a greater extent from those of the growth medium. |
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Authors:
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R G Briggs; J L Glenn |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Lipids Volume: 11 ISSN: 0024-4201 ISO Abbreviation: Lipids Publication Date: 1976 Nov |
Date Detail:
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Created Date: 1977-01-25 Completed Date: 1977-01-25 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0060450 Medline TA: Lipids Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 791-7 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aerobiosis Anaerobiosis Animals Aorta / metabolism*, ultrastructure Cell Line Diglycerides / metabolism Fatty Acids, Nonesterified / metabolism Kinetics Lipid Metabolism* Microscopy, Electron Swine Triglycerides / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Diglycerides; 0/Fatty Acids, Nonesterified; 0/Triglycerides |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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