Document Detail

Lipid accumulation cells derived from porcine aorta and grown under anaerobic conditions.
MedLine Citation:
PMID:  994749     Owner:  NLM     Status:  MEDLINE    
Fibroblast-like cells, derived from porcine aorta, were cultured under aerobic and anaerobic conditions. Light and electron microscopic examinations, lipid composition measurements, and incorporation of radioactive precursors into lipids of these cells were performed. Anaerobically grown cells accumulated oil red O stainable droplets and within 6 hr the triacylglycerol content increased to 4 times the level determined in cells grown under aerobic conditions. This ratio remained constant throughout an additional 12 hr of growth. The fatty acid composition of the triacylglycerols which accumulated under anaerobic conditions differed from the composition of fatty acids in the triacylglycerols present in the growth medium. The cellular unesterified fatty acids of the anaerobically grown cells differed only slightly in composition from the fatty acids in the growth medium, while the unesterfied fatty acids of aerobically grown cells differed to a greater extent from those of the growth medium.
R G Briggs; J L Glenn
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Lipids     Volume:  11     ISSN:  0024-4201     ISO Abbreviation:  Lipids     Publication Date:  1976 Nov 
Date Detail:
Created Date:  1977-01-25     Completed Date:  1977-01-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0060450     Medline TA:  Lipids     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  791-7     Citation Subset:  IM    
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MeSH Terms
Aorta / metabolism*,  ultrastructure
Cell Line
Diglycerides / metabolism
Fatty Acids, Nonesterified / metabolism
Lipid Metabolism*
Microscopy, Electron
Triglycerides / metabolism
Reg. No./Substance:
0/Diglycerides; 0/Fatty Acids, Nonesterified; 0/Triglycerides

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