Document Detail

Lipid profiling reveals tissue-specific differences for ethanolamide lipids in mice lacking fatty acid amide hydrolase.
MedLine Citation:
PMID:  20714818     Owner:  NLM     Status:  MEDLINE    
N-Acylethanolamines (NAE) are fatty acid derivatives, some of which function as endocannabinoids in mammals. NAE metabolism involves common (phosphatidylethanolamines, PEs) and uncommon (N-acylphosphatidylethanolamines, NAPEs) membrane phospholipids. Here we have identified and quantified more than a hundred metabolites in the NAE/endocannabinoid pathway in mouse brain and heart tissues, including many previously unreported molecular species of NAPE. We found that brain tissue of mice lacking fatty acid amide hydrolase (FAAH (-/-)) had elevated PE and NAPE molecular species in addition to elevated NAEs, suggesting that FAAH activity participates in the overall regulation of this pathway. This perturbation of the NAE pathway in brain was not observed in heart tissue of FAAH (-/-) mice, indicating that metabolic regulation of the NAE pathway differs in these two organs and the metabolic enzymes that catabolize NAEs are most likely differentially distributed and/or regulated. Targeted lipidomics analysis, like that presented here, will continue to provide important insights into cellular lipid signaling networks.
Aruna Kilaru; Giorgis Isaac; Pamela Tamura; David Baxter; Scott R Duncan; Barney J Venables; Ruth Welti; Peter Koulen; Kent D Chapman
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2010-08-17
Journal Detail:
Title:  Lipids     Volume:  45     ISSN:  1558-9307     ISO Abbreviation:  Lipids     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-09-02     Completed Date:  2010-12-23     Revised Date:  2014-04-28    
Medline Journal Info:
Nlm Unique ID:  0060450     Medline TA:  Lipids     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  863-75     Citation Subset:  IM    
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MeSH Terms
Amidohydrolases / genetics
Brain / metabolism*
Cannabinoid Receptor Modulators / metabolism*
Ethanolamines / metabolism*
Lipid Metabolism / physiology
Lipids / physiology
Mice, Inbred Strains
Myocardium / metabolism
Phosphatidylethanolamines / metabolism*
Grant Support
P01 AG010485/AG/NIA NIH HHS; P01 AG010485-180009/AG/NIA NIH HHS; P01 AG010485-189006/AG/NIA NIH HHS; P01 AG022550/AG/NIA NIH HHS; P01 AG022550-078407/AG/NIA NIH HHS; P01 AG027956/AG/NIA NIH HHS; P01 AG027956-040003/AG/NIA NIH HHS; P20 RR16475/RR/NCRR NIH HHS; R01 EY014227/EY/NEI NIH HHS; R01 EY014227-04/EY/NEI NIH HHS
Reg. No./Substance:
0/Cannabinoid Receptor Modulators; 0/Ethanolamines; 0/Lipids; 0/N-acylethanolamines; 0/Phosphatidylethanolamines; EC 3.5.-/Amidohydrolases; EC 3.5.1.-/fatty-acid amide hydrolase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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