| Lipid profiling reveals tissue-specific differences for ethanolamide lipids in mice lacking fatty acid amide hydrolase. | |
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MedLine Citation:
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PMID: 20714818 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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N-Acylethanolamines (NAE) are fatty acid derivatives, some of which function as endocannabinoids in mammals. NAE metabolism involves common (phosphatidylethanolamines, PEs) and uncommon (N-acylphosphatidylethanolamines, NAPEs) membrane phospholipids. Here we have identified and quantified more than a hundred metabolites in the NAE/endocannabinoid pathway in mouse brain and heart tissues, including many previously unreported molecular species of NAPE. We found that brain tissue of mice lacking fatty acid amide hydrolase (FAAH (-/-)) had elevated PE and NAPE molecular species in addition to elevated NAEs, suggesting that FAAH activity participates in the overall regulation of this pathway. This perturbation of the NAE pathway in brain was not observed in heart tissue of FAAH (-/-) mice, indicating that metabolic regulation of the NAE pathway differs in these two organs and the metabolic enzymes that catabolize NAEs are most likely differentially distributed and/or regulated. Targeted lipidomics analysis, like that presented here, will continue to provide important insights into cellular lipid signaling networks. |
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Authors:
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Aruna Kilaru; Giorgis Isaac; Pamela Tamura; David Baxter; Scott R Duncan; Barney J Venables; Ruth Welti; Peter Koulen; Kent D Chapman |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. Date: 2010-08-17 |
Journal Detail:
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Title: Lipids Volume: 45 ISSN: 1558-9307 ISO Abbreviation: Lipids Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-09-02 Completed Date: 2010-12-23 Revised Date: 2011-09-13 |
Medline Journal Info:
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Nlm Unique ID: 0060450 Medline TA: Lipids Country: Germany |
Other Details:
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Languages: eng Pagination: 863-75 Citation Subset: IM |
Affiliation:
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Department of Biological Sciences, Center for Plant Lipid Research, University of North Texas, Denton, TX 76203-5017, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amidohydrolases
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genetics Animals Brain / metabolism* Endocannabinoids / metabolism* Ethanolamines / metabolism* Lipid Metabolism / physiology Lipids / physiology Mice Mice, Inbred Strains Myocardium / metabolism Phosphatidylethanolamines / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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P01 AG010485-180009/AG/NIA NIH HHS; P01 AG010485-189006/AG/NIA NIH HHS; P01 AG022550-078407/AG/NIA NIH HHS; P01 AG027956-040003/AG/NIA NIH HHS; P20 RR16475/RR/NCRR NIH HHS; R01 EY014227-04/EY/NEI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Endocannabinoids; 0/Ethanolamines; 0/Lipids; 0/N-acylethanolamines; 0/Phosphatidylethanolamines; EC 3.5.-/Amidohydrolases; EC 3.5.1.-/fatty-acid amide hydrolase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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