Document Detail

Lipase-mediated acidolysis of butteroil with free conjugated linoleic acid in a packed bed reactor.
MedLine Citation:
PMID:  12827703     Owner:  NLM     Status:  MEDLINE    
Acidolysis of butteroil with free conjugated linoleic acid (CLA) was studied in a packed bed reactor containing an immobilized Candida antarctica (fraction B) lipase. Kinetic data were used to develop quantitative reversible rate expressions of the general Michaelis-Menten form that also incorporate a term for first-order deactivation of the enzyme. The extent of incorporation of CLA in the triacylglycerols of butteroil was characterized for reactions carried out at several temperatures (namely 45 degrees, 50 degrees, and 55 degrees C) with different weight ratios of butteroil to CLA (namely 10:1 and 2:1). At the optimum operating temperature of 50 degrees C, similar levels of incorporation of CLA (60% to 85%) were achieved at low space times (<3 h) for both 10:1 and 2:1 (w/w) ratios of butteroil to CLA.
Prima S Sehanputri; Charles G Hill
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Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Validation Studies    
Journal Detail:
Title:  Biotechnology and bioengineering     Volume:  83     ISSN:  0006-3592     ISO Abbreviation:  Biotechnol. Bioeng.     Publication Date:  2003 Sep 
Date Detail:
Created Date:  2003-06-26     Completed Date:  2004-04-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7502021     Medline TA:  Biotechnol Bioeng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  608-17     Citation Subset:  IM    
Copyright Information:
Copyright 2003 Wiley Periodicals, Inc. Biotechnol Bioeng 83: 608-617, 2003.
Department of Chemical Engineering, University of Wisconsin-Madison, 1415 Engineering Drive, Madison, Wisconsin 53706, USA.
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MeSH Terms
Bioreactors / microbiology*
Butter / microbiology*
Candida / growth & development*,  metabolism*
Computer Simulation
Dietary Fats, Unsaturated / metabolism*
Feasibility Studies
Linoleic Acid / biosynthesis*
Lipase / metabolism*
Models, Biological*
Reg. No./Substance:
0/Dietary Fats, Unsaturated; 2197-37-7/Linoleic Acid; 8029-34-3/Butter; EC 3.1.1.-/lipase B, Candida antarctica; EC

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