Document Detail

Lipase-catalyzed modification of rice bran oil to incorporate capric acid.
MedLine Citation:
PMID:  10995376     Owner:  NLM     Status:  MEDLINE    
Capric acid (C10:0) was incorporated into rice bran oil with an immobilized lipase from Rhizomucor miehei as the biocatalyst. Effects of incubation time, substrate mole ratio, enzyme load, and water addition on mole percent incorporation of C10:0 were studied. Transesterification was performed in an organic solvent, hexane, and under solvent-free condition. Pancreatic lipase-catalyzed sn-2 positional analysis and tocopherol analysis were performed before and after enzymatic modification. Products were analyzed by gas-liquid chromatography (GLC) for fatty acid composition. After 24 h of incubation in hexane, there was an average of 26.5 +/- 1.8 mol % incorporation of C10:0 into rice bran oil. The solvent-free reaction produced an average of 24.5 +/- 3.7 mol % capric acid. In general, as the enzyme load, substrate mole ratio, and incubation time increased, the mole percent of capric acid incorporation also increased. Time course reaction indicated C10:0 incorporation increased up to 27.0 mol % at 72 h, for the reaction in hexane, and up to 29.6 mol % at 12 h, for the solvent-free reaction. The highest C10:0 incorporations (53.1 and 43.2 mol %) for the mole ratio experiment occurred at a mole ratio of 1:8 for solvent and solvent-free reactions, respectively. The highest C10:0 incorporation (27.9 mol %) for the reaction in hexane occurred at 10% enzyme load, and the highest incorporation (34.4 mol %) for the solvent-free reaction occurred at 20% enzyme load. Incorporation of C10:0 into rice bran oil declined with the addition of increasing amounts of water after reaching 30.3 mol % at 2% water addition in hexane, and in the solvent-free reaction after reaching 35.9 mol %.
B H Jennings; C C Akoh
Related Documents :
2106316 - Dopachrome conversion factor functions as an isomerase.
7867976 - Reaction of 4-hydroxynonenal with some thiol-containing radioprotective agents or their...
5637366 - Reaction of beta-propiolactone with amino acids and its specificity for methionine.
12713316 - First practical synthesis of formamidine ureas and derivatives.
3754466 - The renal mitochondrial metabolism of 25-hydroxyvitamin d-3: a possible role for phosph...
2778876 - Fatty acid acylation of vaccinia virus proteins.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  48     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2000 Sep 
Date Detail:
Created Date:  2000-11-13     Completed Date:  2000-11-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4439-43     Citation Subset:  IM    
Department of Food Science and Technology, Food Science Building, The University of Georgia, Athens, Georgia 30602-7610, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Decanoic Acids / chemistry*
Lipase / chemistry*
Plant Oils / chemistry*
Reg. No./Substance:
0/Decanoic Acids; 0/Plant Oils; 334-48-5/decanoic acid; 68553-81-1/rice bran oil; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Growth and anthraquinone production of Morinda elliptica cell suspension cultures in a stirred-tank ...
Next Document:  Molecular cloning, characterization, and expression of a cDNA coding Copper/Zinc superoxide dismutas...