Document Detail

Lipase-catalyzed ethanolysis of milk fat with a focus on short-chain fatty acid selectivity.
MedLine Citation:
PMID:  19072544     Owner:  NLM     Status:  MEDLINE    
Mixtures of fatty acid ethyl esters were produced by lipase-catalyzed ethanolysis of milk fat triglycerides. Three commercial immobilized lipases (Lipozyme TL, Lipozyme RM, and Novozym 435) were tested in different reaction conditions with the aim of maximizing the conversion of the short-chain fatty acid fraction of milk fat to flavor ethyl esters. The influence of the reactants molar ratio was investigated, as well as three different reaction media, that is, hexane, CO(2)-expanded liquid (GXL), and the solvent-free mixture. Novozym 435 showed the highest activity in all conditions. This lipase also exhibited selectivity for short-chain fatty acids, which, at short reaction times, resulted in a product mixture richer in short-chain fatty acids than the original milk fat. The highest selectivities were obtained in hexane and in CO(2)-expanded liquid fat, at low ethanol to fat ratios. Using dense CO(2) as the reaction cosolvent is attractive because it results in the largest short-chain fatty acid enrichment in the product mixture, while leaving no residues in the product.
Marta Lubary; Joop H ter Horst; Gerard W Hofland; Peter J Jansens
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  57     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2009-01-07     Completed Date:  2009-02-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  116-21     Citation Subset:  IM    
Department of Process & Energy, Delft University of Technology, Delft, The Netherlands.
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MeSH Terms
Carbon Dioxide
Ethanol / metabolism*
Fatty Acids / analysis
Fatty Acids, Volatile / metabolism*
Lipase / metabolism*
Milk / chemistry*
Substrate Specificity
Triglycerides / metabolism
Reg. No./Substance:
0/Fatty Acids; 0/Fatty Acids, Volatile; 0/Triglycerides; 124-38-9/Carbon Dioxide; 64-17-5/Ethanol; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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