| Lipaemic samples: effective process for lipid reduction using high speed centrifugation compared with ultracentrifugation. | |
| | |
MedLine Citation:
|
PMID: 22141212 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
|
INTRODUCTION: Reducing laboratory errors and improving patient safety is receiving a lot of attention. Lipaemic samples are cause of analytical errors and present challenges for laboratories, particularly for those without ultracentrifuges. Lipaemia can originate from physiological (postprandial metabolism), para-physiological causes (e.g. IV administration of lipids) as well as metabolic disturbances (e.g. hypertriglyceridaemia). MATERIALS AND METHODS: We have evaluated a procedure with 10 native lipaemic sample pools (triglyceride concentration range 11.6-42.7 mmol/L) for the ability to reduce lipid concentration using a high speed micro-centrifuge (double centrifuged at 21.885 x g for 15 min) compared with an ultracentrifuge, and provide accurate results. Results of sodium, creatinine, urate, total protein, lactate dehydrogenase (LD), magnesium and, cholesterol and triglyceride analysis on a Beckman DxC800 analyser are presented. RESULTS: Data from our tertiary level hospital showed approximately 0.7% of the samples received for lipid studies have triglyceride levels > 10 mmol/L which can potentially cause analytical interference. The mean differences from the neat aliquot to the ultracentrifuged and high speed centrifuged sample pools were: cholesterol 4.9 mmol/L and 3.1 mmol/L; and triglycerides 17.4 mmol/L and 15.0 mmol/L respectively. The data confirms high speed centrifugation is almost as effective as ultracentrifugation in lipid reduction. CONCLUSION: The procedure utilized in this study using a high speed micro-centrifuge showed it is effective in reducing lipid levels and provides a suitable alternative to ultracentrifuged samples to provide accurate results. |
| | |
Authors:
|
Goce Dimeski; Brock W Jones |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Biochemia medica : časopis Hrvatskoga društva medicinskih biokemičara / HDMB Volume: 21 ISSN: 1330-0962 ISO Abbreviation: Biochem Med (Zagreb) Publication Date: 2011 |
Date Detail:
|
Created Date: 2011-12-06 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9610305 Medline TA: Biochem Med (Zagreb) Country: Croatia |
Other Details:
|
Languages: eng Pagination: 86-92 Citation Subset: IM |
Affiliation:
|
Department of Chemical Pathology, Pathology Queensland, Princess Alexandra Hospital, Brisbane, Australia. goce_dimeski@health.qld.gov.au |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Effects of hemolysis interferences on routine biochemistry parameters.
Next Document: [Parental presence in the operating room: effect on the quality of anesthetic induction and postoper...