Document Detail


Linoleic acid metabolism and prostaglandin production by cultured bovine pulmonary artery endothelial cells.
MedLine Citation:
PMID:  6751307     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
When bovine pulmonary artery endothelial cells are cultured in a medium supplemented with linoleic acid, their capacity to produce prostacyclin (PGI2) is reduced by about 60%. This reduction occurs when PGI2 formation is stimulated by the addition of either the calcium ionophore A23187 or arachidonic acid. In addition, supplementation with linoleic acid reduced the production of prostaglandin E2 and F2 alpha from 1-14C-arachidonic acid by more than 50%. The capacity of cultured bovine pulmonary vein and aortic endothelial cells to convert extracellular arachidonic acid into PGI2 also was reduced by about 50% when the growth medium was supplemented with linoleic acid. Although bovine pulmonary artery endothelial cells incorporated large amounts of 1-14C-linoleic acid into cellular phospholipids and triglycerides, a maximum of only 2.3% of the radioactivity was converted to arachidonic acid in 24 hours. The most prevalent radioactive metabolite was eicosadienoic acid, the elongation product of linoleic acid. As compared with linoleic acid, the bovine endothelial cells incorporated 30% more 1-14C-arachidonic acid into phospholipids and 60% more into triglycerides. When the growth medium was supplemented with linoleic acid, the percentage of this fatty acid in cellular lipids increased 3- to 4.5-fold and eicosadienoic acid accumulated, accounting for up to 9% of the cellular fatty acids. This increase was accompanied by a 30% to 45% reduction in arachidonic acid. These findings, together with our previous results with human umbilical vein endothelium, suggest that an inability to convert large amounts of linoleic to arachidonic acid and a suppressive effect of linoleic acid enrichment on prostaglandin production may be general properties of endothelial cells.
Authors:
T L Kaduce; A A Spector; R S Bar
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Arteriosclerosis (Dallas, Tex.)     Volume:  2     ISSN:  0276-5047     ISO Abbreviation:  Arteriosclerosis     Publication Date:    1982 Sep-Oct
Date Detail:
Created Date:  1982-12-03     Completed Date:  1982-12-03     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8401388     Medline TA:  Arteriosclerosis     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  380-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Cells, Cultured
Culture Media
Dinoprost
Dinoprostone
Epithelial Cells
Epoprostenol / biosynthesis
Fatty Acids, Unsaturated / metabolism
Linoleic Acid
Linoleic Acids / metabolism*
Prostaglandins / biosynthesis*
Prostaglandins E / biosynthesis
Prostaglandins F / biosynthesis
Pulmonary Artery / cytology*,  metabolism
Radioimmunoassay
Grant Support
ID/Acronym/Agency:
AM 28756/AM/NIADDK NIH HHS; AM25421/AM/NIADDK NIH HHS; HL14230/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Culture Media; 0/Fatty Acids, Unsaturated; 0/Linoleic Acids; 0/Prostaglandins; 0/Prostaglandins E; 0/Prostaglandins F; 2197-37-7/Linoleic Acid; 35121-78-9/Epoprostenol; 363-24-6/Dinoprostone; 551-11-1/Dinoprost

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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