Document Detail

Linoleic acid increases adhesion, chemotaxis, granule release, intracellular calcium mobilisation, MAPK phosphorylation and gene expression in bovine neutrophils.
MedLine Citation:
PMID:  23267746     Owner:  NLM     Status:  Publisher    
Neutrophils are critical to the innate immune response; therefore, the proper function of neutrophils is critical to avoid the development of certain diseases. Linoleic acid, a polyunsaturated long-chain fatty acid, is one of the most abundant long-chain fatty acids found in the plasma of cows after giving birth. In this study, we evaluated the effects of linoleic acid treatment on bovine neutrophil adhesion, chemotaxis, metalloproteinase (MMP)-9 release, CD11b expression, intracellular calcium mobilisation, mitogen-activating protein kinase (MAPK) phosphorylation and COX-2 and IL-8 expression. Bovine neutrophils isolated from healthy heifers were incubated with different concentrations of linoleic acid, and then neutrophil responses were evaluated. Our results show that the treatment of neutrophils with 100μM linoleic acid increased their adhesion to the bovine endothelial cell line CPA47. The results of a transwell migration assay revealed that linoleic acid could also promote the chemotaxis of bovine neutrophils. Furthermore, linoleic acid treatment increased MMP-9 activity and CD11b cell surface expression in neutrophils. Fifty and 100μM linoleic acid also increased intracellular calcium mobilisation in neutrophils loaded with Fluo-4 AM dye. Linoleic acid also rapidly (2-5min) stimulated the phosphorylation of ERK1/2 and p38 MAPK as evaluated by immunoblot. Finally, COX-2 and IL-8 mRNA expression increased after 2h of linoleic acid treatment. In conclusion, linoleic acid stimulates adhesion, chemotaxis, granule release and intracellular responses in bovine neutrophils.
Jaqueline Mena; Carolina Manosalva; Ruben Ramirez; Lhia Chandia; Daniel Carroza; Anitsi Loaiza; Rafael A Burgos; Maria A Hidalgo
Related Documents :
22002626 - Reduced expression of fata thioesterases in arabidopsis affects the oil content and fat...
9420796 - Radionuclide assessment of myocardial fatty acid metabolism by pet and spect.
2979846 - Polyenoic fatty acid deprivation of juvenile cats modulates 3h-dopamine release from pr...
19666956 - Emission of volatile aldehydes and ketones from wood pellets under controlled conditions.
16137726 - Characterization of cross-linked hydroxycinnamic acid amides isolated from potato commo...
3413026 - Intestinal absorption of chloral hydrate, free trichloroethanol and trichloroacetic aci...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-5
Journal Detail:
Title:  Veterinary immunology and immunopathology     Volume:  -     ISSN:  1873-2534     ISO Abbreviation:  Vet. Immunol. Immunopathol.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-26     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8002006     Medline TA:  Vet Immunol Immunopathol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier B.V. All rights reserved.
Laboratory of Molecular Pharmacology, Institute of Pharmacology, Faculty of Veterinary Science, Universidad Austral de Chile, Valdivia, Chile; Department of Biology, Universidad de Nariño, Pasto, Colombia.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  A new equation to estimate basal energy expenditure of patients with diabetes.
Next Document:  Secondary and Tertiary Hyperparathyroidism.