| Linoleic acid and butyrate synergize to increase Bcl-2 levels in colonocytes. | |
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MedLine Citation:
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PMID: 20232381 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The biological properties of polyunsaturated fatty acid (PUFA) classes have been the source of much contention. For example, n-3 PUFA are chemoprotective, whereas n-6 PUFA may promote tumor development. Since dietary components can have combinatorial effects, we further examined the apoptotic properties of n-3 or n-6 fatty acids when combined with different fiber sources. Mice were fed diets supplemented with either fish oil (FO; enriched in n-3 PUFA) or corn oil (CO; enriched in n-6 PUFA) and nonfermentable (cellulose) or fermentable (pectin) fiber sources. In complementary experiments, immortalized young adult mouse colonic (YAMC) cells were treated with docosahexaenoic acid (DHA; 22:6n-3) or linoleic acid (LA; 18:2n-6) with or without butyrate. Mice fed a FO and pectin diet had significantly (p < 0.05) increased levels of apoptosis in colonocytes compared to all other diets. Similarly, apoptosis was highly induced in DHA and butyrate cotreated YAMC cells. In contrast, in both YAMC and mouse models, LA/CO with butyrate/pectin treatment reduced apoptosis and enhanced expression of bcl-2. The LA and butyrate induced antiapoptotic phenotype was reversed by knocking down bcl-2 using targeted siRNA. In comparison, overexpression of bcl-2 blocked the proapoptotic effect of DHA and butyrate. These data provide new mechanistic insights into the regulation of apoptosis by dietary PUFA and fiber. |
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Authors:
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Harmony F Turk; Satya S Kolar; Yang-Yi Fan; Caitlin A Cozby; Joanne R Lupton; Robert S Chapkin |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: International journal of cancer. Journal international du cancer Volume: 128 ISSN: 1097-0215 ISO Abbreviation: Int. J. Cancer Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2010-10-28 Completed Date: 2010-12-30 Revised Date: 2012-01-04 |
Medline Journal Info:
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Nlm Unique ID: 0042124 Medline TA: Int J Cancer Country: United States |
Other Details:
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Languages: eng Pagination: 63-71 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 UICC. |
Affiliation:
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Program in Integrative Nutrition and Complex Diseases, Texas A&M University, College Station, TX, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis / drug effects Butyrates / administration & dosage, pharmacology* Cells, Cultured Cellulose / administration & dosage, pharmacology Colon / cytology, drug effects*, metabolism Corn Oil / administration & dosage, pharmacology Docosahexaenoic Acids / pharmacology Drug Synergism Fish Oils / administration & dosage, pharmacology Gene Expression / drug effects Immunoblotting In Situ Nick-End Labeling Intestinal Mucosa / drug effects, metabolism Linoleic Acid / administration & dosage, pharmacology* Mice Mice, Inbred C57BL Pectins / administration & dosage, pharmacology Proto-Oncogene Proteins c-bcl-2 / genetics, metabolism* RNA Interference Reverse Transcriptase Polymerase Chain Reaction |
| Grant Support | |
ID/Acronym/Agency:
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CA59034/CA/NCI NIH HHS; P30ES09106/ES/NIEHS NIH HHS; R01 CA059034-17/CA/NCI NIH HHS; R01 CA129444-04/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Butyrates; 0/Fish Oils; 0/Pectins; 0/Proto-Oncogene Proteins c-bcl-2; 2197-37-7/Linoleic Acid; 25167-62-8/Docosahexaenoic Acids; 8001-30-7/Corn Oil; 9000-69-5/pectin; 9004-34-6/Cellulose |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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