| Linoleic acid, but not cortisol, stimulates accumulation of casein by mouse mammary epithelial cells in serum-free collagen gel culture. | |
| | |
MedLine Citation:
|
PMID: 3317407 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A two-step culture system has been developed to analyze the role of hormones in casein accumulation by mammary epithelial cells obtained from adrenalectomized and ovariectomized adult virgin mice. In the first step cells are grown inside collagen gel in medium containing insulin, epidermal growth factor (EGF), and linoleic acid for 9 days; these conditions stimulate very little casein accumulation. Following this growth phase the gels are released to float in medium containing insulin, prolactin, and linoleic acid. During this second phase the mammary cells will accumulate large amounts of casein, but only in the simultaneous presence of insulin, prolactin, and linoleic acid; in the absence of linoleic acid casein accumulation is greatly reduced. The casein accumulation is not dependent on the presence of the glucocorticoid cortisol and will occur in spite of the presence of the antiglucocorticoid agent RU 38 486. To determine if the response to cortisol observed in organ culture by other investigators might be mediated by stromal cells, epithelial cells were grown in collagen gel under fatty acid-free conditions and then cocultured with explants of mammary fat pads from adult virgin mice with or without mammary parenchyma. The cocultures were performed in fatty acid-free medium containing insulin and prolactin with or without cortisol. In the majority of experiments the mammary epithelial cells in the collagen gel accumulate more casein in the presence of cortisol than in its absence, irrespective of the presence of mammary parenchyma in the explant. Thus, mammary epithelial cells are directly dependent on insulin and prolactin for casein accumulation and indirectly dependent on cortisol by means of its effect on the stromal cells. This cortisol effect may be to cause release into the medium of linoleic acid or a metabolic product of linoleic acid from the stromal cells. |
| | |
Authors:
|
B K Levay-Young; G K Bandyopadhyay; S Nandi |
Related Documents
:
|
3702617 - Simultaneous analysis of low plasma levels of deuterium-labeled saturated and unsaturat... 12940547 - On the specificity of lipid hydroperoxide fragmentation by fatty acid hydroperoxide lya... 1143027 - The incorporation of orally fed radioactive gamma-linolenic acid and linoleic acid into... 3396317 - Characterization of the constituent fatty acids in phosphatidylinositol during early de... 23432317 - Effect of stilbene and chalcone scaffolds incorporation in clofibric acid on ppara agon... 7356647 - The purification and partial characterization of human somatomedin c. |
Publication Detail:
|
Type: In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 84 ISSN: 0027-8424 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 1987 Dec |
Date Detail:
|
Created Date: 1988-01-07 Completed Date: 1988-01-07 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 8448-52 Citation Subset: IM |
Affiliation:
|
Cancer Research Laboratory, University of California, Berkeley 94720. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Caseins / metabolism* Collagen Epithelium / metabolism Estrenes / pharmacology Gels Hydrocortisone / pharmacology* Insulin / pharmacology Linoleic Acid Linoleic Acids / pharmacology* Mammary Glands, Animal / cytology, metabolism* Mice Mifepristone Prolactin / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
|
CA05388/CA/NCI NIH HHS; CA09041/CA/NCI NIH HHS; CA40160/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Caseins; 0/Estrenes; 0/Gels; 0/Linoleic Acids; 11061-68-0/Insulin; 2197-37-7/Linoleic Acid; 50-23-7/Hydrocortisone; 84371-65-3/Mifepristone; 9002-62-4/Prolactin; 9007-34-5/Collagen |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Correlation between the 32-kDa sigma factor levels and in vitro expression of Escherichia coli heat ...
Next Document: Autocrine stimulation after transfer of the granulocyte/macrophage colony-stimulating factor gene an...