Document Detail

Linking phospholipase A2 to phospholipid turnover and prostaglandin synthesis in mast cell granules.
MedLine Citation:
PMID:  1900237     Owner:  NLM     Status:  MEDLINE    
Rapid incorporation of exogenous arachidonic acid into phospholipid has been detected in conjunction with eicosanoid synthesis by purified mast cell granules [Chock, S. P. & Schmauder-Chock, E. A. (1988) Biochem. Biophys. Res. Commun. 156, 1308-1315]. The species of phospholipid formed has now been identified primarily as phosphatidylinositol. A calcium-dependent phospholipase A2 has also been detected in the secretory granule. This enzyme, like the cyclooxygenase [Schmauder-Chock, E. A. & Chock, S. P. (1989) J. Histochem. Cytochem. 37, 1319-1328], appears to bind tightly to the granule matrix components. It is heat resistant and requires millimolar concentrations of calcium for optimal activity. It prefers phosphatidylinositol over phosphatidylcholine as substrate. Since the granule contains a large amount of phospholipid, the action of this phospholipase A2 can provide the required substrate for the arachidonic acid cascade. These findings provide the basis for linking phospholipase A2 to the production of eicosanoids during granule exocytosis. Since the granule also contains both an active acylating system that can rapidly reacylate lysophosphatidylinositol to form phosphatidylinositol, and an active phospholipase A2 which hydrolyzes phosphatidylinositol, a rapid turnover involving the fatty acid at the sn-2 position of phosphatidylinositol may occur. These findings are consistent with our postulation that the secretory granule is the source and/or the cause of many of the early biochemical events associated with the process of stimulus-secretion coupling.
S P Chock; S G Rhee; L C Tang; E A Schmauder-Chock
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  European journal of biochemistry / FEBS     Volume:  195     ISSN:  0014-2956     ISO Abbreviation:  Eur. J. Biochem.     Publication Date:  1991 Feb 
Date Detail:
Created Date:  1991-04-08     Completed Date:  1991-04-08     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0107600     Medline TA:  Eur J Biochem     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  707-13     Citation Subset:  IM    
Division of Experimental Therapeutics, Walter Reed Army Institute of Research, Washington, DC.
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MeSH Terms
Arachidonic Acid
Arachidonic Acids / metabolism
Carbon Radioisotopes
Cytoplasmic Granules / metabolism*
Mast Cells / metabolism*
Phospholipases A / metabolism*
Phospholipases A2
Phospholipids / metabolism*
Prostaglandins / biosynthesis*
Rats, Inbred Strains
Reg. No./Substance:
0/Arachidonic Acids; 0/Carbon Radioisotopes; 0/Phospholipids; 0/Prostaglandins; 506-32-1/Arachidonic Acid; EC 3.1.1.-/Phospholipases A; EC A2

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