Document Detail


Linking NE1545 gene expression with cell volume changes in Nitrosomonas europaea cells exposed to aromatic hydrocarbons.
MedLine Citation:
PMID:  21106218     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
Nitrosomonas europaea, a model ammonia oxidizing bacterium, was exposed to a wide variety of aromatic hydrocarbons in 3 h batch assays. The expression of NE1545, a phenol sentinel gene involved in fatty acid metabolism, was monitored via quantitative real-time polymerase chain reaction (qRT-PCR) and a Coulter Counter technique was used to monitor changes in cell volume. Decreases in cell volume and NE1545 gene expression correlated strongly with exposure to aromatic hydrocarbons that possessed a single polar group substitution (e.g. phenol and aniline). Aromatic hydrocarbons that contain no polar group substitutions (e.g. toluene) or multiple polar group substitutions (e.g. p-hydroquinone) caused negligible changes in NE1545 expression and cell volume. The oxidation of aromatic hydrocarbons by N. europaea from configurations without a single polar group to one with two polar groups (e.g. p-cresol oxidized to 4-hydroxybenzyl alcohol) and from configurations with no polar groups to one with a single polar group (e.g. ethylbenzene oxidized to 4-ethylphenol) greatly influenced NE1545 gene expression and observed changes in cell volume. Nitrification inhibition in N. europaea by the aromatic hydrocarbons was found to be completely reversible; however, the decreases in cell volume were not reversible suggesting a physical change in cell membrane composition. Ammonia monooxygenase blocking studies showed that the chemical exposure that was responsible for the cell volume decrease and up-regulation in gene expression and not the observed inhibition. N. europaea is the first bacterium shown to experience significant changes in cell volume when exposed to μM concentrations of aromatic hydrocarbons, three orders of magnitude lower than previous studies with other bacteria.
Authors:
Tyler S Radniecki; Caslin A Gilroy; Lewis Semprini
Related Documents :
15166628 - Cholesteryl butyrate solid lipid nanoparticles as a butyric acid pro-drug: effects on c...
7803488 - Lack of inhibition by dideoxy-forskolin and verapamil of dids-sensitive volume-activate...
17101138 - Dysfunction of regulatory volume increase is a key component of apoptosis.
6806478 - Effects of glutaraldehyde fixative osmolarities on smooth muscle cell volume, and osmot...
22358098 - Oleanolic acid derivative methyl 3,11-dioxoolean-12-en-28-olate targets multidrug resis...
3189868 - Development of arteries in embryonic chick bone marrow with special reference to the ap...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-11-23
Journal Detail:
Title:  Chemosphere     Volume:  82     ISSN:  1879-1298     ISO Abbreviation:  Chemosphere     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2010-12-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0320657     Medline TA:  Chemosphere     Country:  England    
Other Details:
Languages:  eng     Pagination:  514-20     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Ltd. All rights reserved.
Affiliation:
Oregon State University, Corvallis, 97331, USA. tyler.radniecki@oregonstate.edu
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Direct photodegradation of carbamazepine followed by micellar electrokinetic chromatography and mass...
Next Document:  Physiological effects of arsenic in the lichen Xanthoria parietina (L.) Th. Fr.