Document Detail


Linkage mapping of the highly informative DNA marker D21S156 to human chromosome 21 using a polymorphic GT dinucleotide repeat.
MedLine Citation:
PMID:  1979059     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A (GT)n repeat within the anonymous DNA sequence D21S156 was shown to be highly polymorphic in DNA from members of the 40 CEPH families. At least 12 alleles of this locus were recognized by electrophoresis on polyacrylamide gels of DNA amplified by the polymerase chain reaction (PCR) using primers flanking the (GT)n repeat. The polymorphism information content was 0.82. PCR amplification of DNA from somatic cell hybrid lines mapped D21S156 to human chromosome 21 and linkage analysis localized this marker close to the loci ETS2, D21S3, and HMG14 on chromosomal band 21q22.3. This polymorphism is highly informative and can serve as an anchor locus for human chromosome 21.
Authors:
J G Lewis; J L Weber; M B Petersen; S A Slaugenhaupt; A Kwitek; P E May; A C Warren; A Chakravarti; S E Antonarakis
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Genomics     Volume:  8     ISSN:  0888-7543     ISO Abbreviation:  Genomics     Publication Date:  1990 Oct 
Date Detail:
Created Date:  1991-01-16     Completed Date:  1991-01-16     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8800135     Medline TA:  Genomics     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  400-2     Citation Subset:  IM    
Affiliation:
Center for Medical Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
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MeSH Terms
Descriptor/Qualifier:
Alleles
Base Sequence
Chromosome Mapping
Chromosomes, Human, Pair 21*
Genetic Markers*
Humans
Lod Score
Molecular Sequence Data
Pedigree
Polymorphism, Restriction Fragment Length*
Grant Support
ID/Acronym/Agency:
GM33771/GM/NIGMS NIH HHS; GM41773/GM/NIGMS NIH HHS; HD00774/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Genetic Markers

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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