Document Detail


Linkage of cell cycle kinetics between embryonic and adult stroke models: an analytical approach.
MedLine Citation:
PMID:  17141877     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In the adult brain, neurogenesis occurs in the subventricular zone (SVZ) of the lateral ventricle. The proliferating population and the cell cycle kinetics in the ventricular zone regulate cortical neurogenesis during the development. Using the embryonic model, we investigated kinetics of SVZ cells in adult rats after stroke, incorporating migration of SVZ cells to the ischemic boundary. Concurrent linear regressions were considered through iteration to improve precision of parameter estimation. We found no model-fit difference in stroke with and without the migration (p=0.31), suggesting no migration effect on assessment of the cell kinetics. Stroke increased SVZ cell proliferation (20% in non-stroke and 31% in stroke p<0.01). Cell cycle durations in stroke were reduced for the total cycle length (19h for non-stroke and 15.3h for stroke, p<0.05), in G1 phase (12.6 h for non-stroke and 9.6 h for stroke, p<0.01), but were the same in S, M2, and in G2 phases compared to non-stroke, indicating that stroke reduces the total cell cycle length, specially in G1 phase. We conclude that cell cycle kinetic models for cortical development can be adapted to the kinetics of adult SVZ cells after stroke. The analytical approach may be useful for studying neural progenitor cell proliferation under different treatments.
Authors:
Mei Lu; Rui Lan Zhang; Zheng Gang Zhang; James J Yang; Michael Chopp
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2006-12-04
Journal Detail:
Title:  Journal of neuroscience methods     Volume:  161     ISSN:  0165-0270     ISO Abbreviation:  J. Neurosci. Methods     Publication Date:  2007 Apr 
Date Detail:
Created Date:  2007-03-30     Completed Date:  2007-06-12     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7905558     Medline TA:  J Neurosci Methods     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  323-30     Citation Subset:  IM    
Affiliation:
Department of Biostatistics and Research Epidemiology, Henry Ford Health Sciences Center, One Ford Place, Ste. 3E, Detroit, MI 48202, USA. mlu1@hfhs.org
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MeSH Terms
Descriptor/Qualifier:
Aging / pathology*
Animals
Brain / embryology,  pathology
Cell Cycle*
Cells, Cultured
Computer Simulation
Disease Models, Animal
Kinetics
Models, Neurological*
Neurons / pathology*
Rats
Stroke / embryology*,  pathology*
Grant Support
ID/Acronym/Agency:
P01 NS42345/NS/NINDS NIH HHS; P01-NS23393/NS/NINDS NIH HHS; R01HL6476/HL/NHLBI NIH HHS; R01NS38292/NS/NINDS NIH HHS

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