Document Detail

Linkage between the bacterial acid stress and stringent responses: the structure of the inducible lysine decarboxylase.
MedLine Citation:
PMID:  21278708     Owner:  NLM     Status:  MEDLINE    
The Escherichia coli inducible lysine decarboxylase, LdcI/CadA, together with the inner-membrane lysine-cadaverine antiporter, CadB, provide cells with protection against mild acidic conditions (pH∼5). To gain a better understanding of the molecular processes underlying the acid stress response, the X-ray crystal structure of LdcI was determined. The structure revealed that the protein is an oligomer of five dimers that associate to form a decamer. Surprisingly, LdcI was found to co-crystallize with the stringent response effector molecule ppGpp, also known as the alarmone, with 10 ppGpp molecules in the decamer. ppGpp is known to mediate the stringent response, which occurs in response to nutrient deprivation. The alarmone strongly inhibited LdcI enzymatic activity. This inhibition is important for modulating the consumption of lysine in cells during acid stress under nutrient limiting conditions. Hence, our data provide direct evidence for a link between the bacterial acid stress and stringent responses.
Usheer Kanjee; Irina Gutsche; Eftichia Alexopoulos; Boyu Zhao; Majida El Bakkouri; Guillaume Thibault; Kaiyin Liu; Shaliny Ramachandran; Jamie Snider; Emil F Pai; Walid A Houry
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-01-28
Journal Detail:
Title:  The EMBO journal     Volume:  30     ISSN:  1460-2075     ISO Abbreviation:  EMBO J.     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-03-02     Completed Date:  2011-05-05     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  8208664     Medline TA:  EMBO J     Country:  England    
Other Details:
Languages:  eng     Pagination:  931-44     Citation Subset:  IM    
Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada.
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MeSH Terms
Amino Acid Sequence
Amino Acid Transport Systems / chemistry*,  metabolism
Antiporters / chemistry*,  metabolism
Carboxy-Lyases / chemistry*,  metabolism
Catalytic Domain
Crystallography, X-Ray
Enzyme Inhibitors / pharmacology
Escherichia coli / enzymology*
Escherichia coli Proteins / chemistry*,  metabolism
Hydrogen-Ion Concentration
Molecular Sequence Data
Protein Conformation
Protein Multimerization
Stress, Physiological*
Grant Support
MOP-67210//Canadian Institutes of Health Research
Reg. No./Substance:
0/Amino Acid Transport Systems; 0/Antiporters; 0/CadB protein, E Coli; 0/Enzyme Inhibitors; 0/Escherichia coli Proteins; EC 4.1.1.-/Carboxy-Lyases; EC decarboxylase

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