Document Detail

Linezolid and vancomycin decrease the therapeutic effect of methylene blue-photodynamic therapy in a mouse model of MRSA bacterial arthritis.
MedLine Citation:
PMID:  23311407     Owner:  NLM     Status:  MEDLINE    
We previously reported that photodynamic therapy (PDT) using intra-articular methylene blue (MB) could be used to treat arthritis in mice caused by bioluminescent methicillin-resistant Staphylococcus aureus (MRSA) either in a therapeutic or in a preventative mode. PDT accumulated neutrophils into the mouse knee via activation of chemoattractants such as inflammatory cytokines or chemokines. In this study, we asked whether PDT combined with antibiotics used for MRSA could provide added benefit in controlling the infection. We compared MB-PDT alone, systemic administration of either linezolid (LZD) alone or vancomycin (VCM) alone or the combination of PDT with either LZD or VCM. Real-time noninvasive imaging was used to serially follow the progress of the infection. PDT alone was the most effective, whereas LZD alone was ineffective and VCM alone showed some benefit. Surprisingly the addition of LZD or VCM reduced the therapeutic effect of PDT alone (P < 0.05). Considering that PDT in this mouse model stimulates neutrophils to be antibacterial rather than actively killing the bacteria, we propose that LZD and VCM might inhibit the activation of inflammatory cytokines without eradicating the bacteria, and thereby reduce the therapeutic effect of PDT.
Masamitsu Tanaka; Pawel Mroz; Tianhong Dai; Liyi Huang; Yuji Morimoto; Manabu Kinoshita; Yasuo Yoshihara; Nariyoshi Shinomiya; Shuhji Seki; Koichi Nemoto; Michael R Hamblin
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2013-02-19
Journal Detail:
Title:  Photochemistry and photobiology     Volume:  89     ISSN:  1751-1097     ISO Abbreviation:  Photochem. Photobiol.     Publication Date:    2013 May-Jun
Date Detail:
Created Date:  2013-04-25     Completed Date:  2013-11-26     Revised Date:  2014-05-07    
Medline Journal Info:
Nlm Unique ID:  0376425     Medline TA:  Photochem Photobiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  679-82     Citation Subset:  IM    
Copyright Information:
© 2013 Wiley Periodicals, Inc. Photochemistry and Photobiology © 2013 The American Society of Photobiology.
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MeSH Terms
Acetamides / pharmacology*
Anti-Bacterial Agents / pharmacology*
Arthritis, Experimental / drug therapy,  immunology,  pathology
Arthritis, Infectious / drug therapy*,  immunology,  pathology
Cytokines / biosynthesis,  immunology
Drug Antagonism
Drug Therapy, Combination
Injections, Intra-Articular
Joint Capsule / drug effects,  immunology,  pathology
Methicillin-Resistant Staphylococcus aureus*
Methylene Blue / pharmacology*
Mice, Inbred C57BL
Neutrophils / drug effects,  immunology,  pathology
Oxazolidinones / pharmacology*
Photosensitizing Agents / pharmacology*
Staphylococcal Infections / drug therapy*,  immunology,  pathology
Vancomycin / pharmacology*
Grant Support
Reg. No./Substance:
0/Acetamides; 0/Anti-Bacterial Agents; 0/Cytokines; 0/Oxazolidinones; 0/Photosensitizing Agents; 165800-03-3/linezolid; 6Q205EH1VU/Vancomycin; T42P99266K/Methylene Blue

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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