Document Detail

Lineal energy-based evaluation of oxidative DNA damage induced by proton beams and X-rays.
MedLine Citation:
PMID:  22901337     Owner:  NLM     Status:  MEDLINE    
PURPOSE: To determine the oxidative capabilities of proton beams compared to X-rays based on lineal energy (y).
MATERIALS AND METHODS: Microdosimetry was used to determine y-values of 155 MeV protons. Salmon testes deoxyribonucleic acid (ST-DNA) in solution and human tumor cells (MOLT-4) were irradiated with 200 kV X-rays (X) or 155 MeV protons at their plateau (P) and near their Bragg-peak (B). 8-Hydroxydeoxyguanosine (8-OHdG) production was determined by high performance liquid chromatography. Double-strand breaks (DSB) in ST-DNA were evaluated by agarose gel electrophoresis and DSB in cell nuclei were evaluated by immunocytochemical analysis of phosphorylated histone H2AX (γH2AX) foci. Edaravone was used as a radical scavenger.
RESULTS: 8-OHdG yields in ST-DNA were significantly higher with X than with P or B, and they were significantly higher with P than with B. DSB yields in ST-DNA were higher with P than with B or X, although not statistically significant, and were nearly equal with B and X. Although γH2AX foci formation in MOLT-4 cells after each irradiation type was nearly identical, the addition of edaravone significantly inhibited foci formation only with X.
CONCLUSIONS: Our results indicated that radical-induced indirect DNA damage was significantly lower with proton beams than with X-rays.
Zhengshan Hong; Yuki Kase; Takashi Moritake; Ariungerel Gerelchuluun; Lue Sun; Kenshi Suzuki; Toshiyuki Terunuma; Kiyoshi Yasuoka; Hiroaki Kumada; Kazunori Anzai; Hideyuki Sakurai; Takeji Sakae; Koji Tsuboi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-09-13
Journal Detail:
Title:  International journal of radiation biology     Volume:  89     ISSN:  1362-3095     ISO Abbreviation:  Int. J. Radiat. Biol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-01-02     Completed Date:  2013-02-25     Revised Date:  2013-11-12    
Medline Journal Info:
Nlm Unique ID:  8809243     Medline TA:  Int J Radiat Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  36-43     Citation Subset:  IM; S    
Proton Medical Research Center, Graduate School of Comprehensive Human Sciences, University of Tsukuba.
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MeSH Terms
Cell Line, Tumor
DNA Breaks, Double-Stranded / radiation effects
DNA Damage*
Deoxyguanosine / analogs & derivatives,  metabolism
Histones / metabolism
Linear Energy Transfer / radiation effects*
Oxidative Stress / radiation effects*
Photons / adverse effects
Protons / adverse effects*
X-Rays / adverse effects
Reg. No./Substance:
0/8-hydroxy-2'-deoxyguanosine; 0/H2AFX protein, human; 0/Histones; 0/Protons; 961-07-9/Deoxyguanosine
Erratum In:
Int J Radiat Biol. 2013 Oct;89(10):888

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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