Document Detail


Lineage mapping the pre-implantation mouse embryo by two-photon microscopy, new insights into the segregation of cell fates.
MedLine Citation:
PMID:  21539832     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The first lineage segregation in the pre-implantation mouse embryo gives rise to cells of the inner cell mass and the trophectoderm. Segregation into these two lineages during the 8-cell to 32-cell stages is accompanied by a significant amount of cell displacement, and as such it has been difficult to accurately track cellular behavior using conventional imaging techniques. Consequently, how cellular behaviors correlate with cell fate choices is still not fully understood. To achieve the high spatial and temporal resolution necessary for tracking individual cell lineages, we utilized two-photon light-scanning microscopy (TPLSM) to visualize and follow every cell in the embryo using fluorescent markers. We found that cells undergoing asymmetric cell fate divisions originate from a unique population of cells that have been previously classified as either outer or inner cells. This imaging technique coupled with a tracking algorithm we developed allows us to show that these cells, which we refer to as intermediate cells, share features of inner cells but exhibit different dynamic behaviors and a tendency to expose their cell surface in the mouse embryo between the fourth and fifth cleavages. We provide an accurate description of the correlation between cell division order and cell fate, and demonstrate that cell cleavage angle is a more accurate indicator of cellular polarity than cell fate. Our studies demonstrate the utility of two-photon imaging in answering questions in the pre-implantation field that have previously been difficult or impossible to address. Our studies provide a framework for the future use of specific markers to track cell fate molecularly and with high accuracy.
Authors:
Katie McDole; Yuan Xiong; Pablo A Iglesias; Yixian Zheng
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-04-22
Journal Detail:
Title:  Developmental biology     Volume:  355     ISSN:  1095-564X     ISO Abbreviation:  Dev. Biol.     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-06-20     Completed Date:  2011-09-09     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  239-49     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier Inc. All rights reserved.
Affiliation:
Department of Biology, Johns Hopkins University, 3400 N. Charles St., Baltimore, MD 21218, USA.
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MeSH Terms
Descriptor/Qualifier:
Algorithms*
Animals
Blastocyst*
Cell Differentiation / physiology*
Cell Lineage / physiology*
Embryonic Development / physiology*
Green Fluorescent Proteins / genetics,  metabolism
Histones / genetics,  metabolism
Image Processing, Computer-Assisted
Mice
Mice, Transgenic
Microscopy, Fluorescence / methods
Grant Support
ID/Acronym/Agency:
R21 AA 20060-01/AA/NIAAA NIH HHS; R21 AA020060/AA/NIAAA NIH HHS; S10 RR025505-01/RR/NCRR NIH HHS; //Howard Hughes Medical Institute; //Howard Hughes Medical Institute
Chemical
Reg. No./Substance:
0/Histones; 147336-22-9/Green Fluorescent Proteins
Comments/Corrections

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