Document Detail

Lineage analysis of newly generated neurons in organotypic culture of rat hippocampus.
MedLine Citation:
PMID:  21145363     Owner:  NLM     Status:  Publisher    
New neurons are continuously generated in the hippocampus at the subgranular zone of the dentate granule cell layer throughout life. However, the lineage of newly generated neurons is unknown in detail. Here, using a retrovirus vector encoding EGFP, we labeled proliferating cells in an organotypic slice culture of the postnatal hippocampus of rat, and tracked their descendents over a long period. At 28 days post-inoculation, the phenotypes of the cells were immunohistochemically identified using specific antibodies to cell-type markers such as HuC/D (pan-neuronal marker), GFAP (astrocyte marker), Prox1 (dentate granule cell marker) or NeuN (mature neuronal marker). We found that the cells were mostly GFAP-negative in the HuC/D-positive lineages. The EGFP-expressing cells were often untraceable shortly after cell division in the HuC/D-positive lineages. The postmitotic periods of these cells distributed between 2 and 14 days. For the lineages expressing both Prox1 and NeuN the newborn cells became untraceable in a similar period (2-10 days). It is suggested that the newly generated neurons differentiate to mature dentate granule cells in the slice culture once they have survived over this critical traceability period.
Jun Yokose; Toru Ishizuka; Takeshi Yoshida; Jun Aoki; Yoshio Koyanagi; Hiromu Yawo
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2010-12-8
Journal Detail:
Title:  Neuroscience research     Volume:  -     ISSN:  1872-8111     ISO Abbreviation:  -     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2011-1-3     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8500749     Medline TA:  Neurosci Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2010 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.
Department of Developmental Biology and Neuroscience, Tohoku University Graduate School of Life Sciences, Sendai 980-8577, Japan; JST, CREST, Tokyo 102-0075, Japan; Tohoku University Basic and Translational Research Center for Global Brain Science, Sendai 980-8575, Japan.
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