| Limiting dilution analysis of the human T cell response to mycobacterial antigens from BCG vaccinated individuals and leprosy patients. | |
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MedLine Citation:
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PMID: 3308217 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The number of peripheral blood T lymphocytes responding to soluble mycobacterial antigens from Mycobacterium tuberculosis purified protein derivative (PPD) and M. leprae (MLS) was estimated by limiting dilution analysis. Antigen-induced lymphocyte activation was measured by means of [3H]TdR incorporation on day 10 of culture in the presence of suboptimal concentrations of interleukin 2 (IL-2). In the peripheral blood of BCG-vaccinated individuals from the UK, the frequency of T lymphocytes responding to PPD was 1.5 to 4 times greater than to MLS. Frequencies between 1/1970 and 1/13, 982 were observed in response to PPD and between 1/4097 and 1/24, 717 in response to MLS. A proportion of cells in the peripheral blood were also observed to respond to IL-2 only. The frequency of cells observed in limiting dilution analysis for PPD and MLS reflected the relative amounts of proliferation to these two antigens in bulk culture lymphocyte transformation tests. Use of PPD-specific T cell lines suggested that the responsiveness observed to M. leprae antigens in BCG-vaccinated individuals was due to cross-reactivity with antigens shared with M. bovis BCG. In tuberculoid leprosy, the frequency of peripheral blood T lymphocytes responding to M. leprae antigens was either greater than or similar to the frequency of T cells responding to PPD. In contrast, limiting dilution analysis of T lymphocytes from the peripheral blood of lepromatous leprosy patients revealed the complex regulatory heterogeneity of this group. In some patients M. leprae responsive T cells were detected in the presence of exogenous IL-2. |
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Authors:
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S J Brett; A E Kingston; M J Colston |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Clinical and experimental immunology Volume: 68 ISSN: 0009-9104 ISO Abbreviation: Clin. Exp. Immunol. Publication Date: 1987 Jun |
Date Detail:
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Created Date: 1987-11-09 Completed Date: 1987-11-09 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0057202 Medline TA: Clin Exp Immunol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 510-20 Citation Subset: IM |
Affiliation:
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Laboratory for Leprosy and Mycobacterial Research, National Institute for Medical Research, London, UK. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, Bacterial
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immunology* BCG Vaccine / immunology* Cross Reactions Female Humans Interleukin-2 / immunology Leprosy / immunology* Leukocyte Count Lymphocyte Activation Male Mycobacterium leprae / immunology* T-Lymphocytes / immunology* Tuberculin / immunology |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Bacterial; 0/BCG Vaccine; 0/Interleukin-2; 0/Tuberculin |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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