| Limited usefulness of the PFA-100 for the monitoring of ADP receptor antagonists--in vitro experience. | |
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MedLine Citation:
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PMID: 15061376 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have evaluated the usefulness of the PFA-100 system (collagen/ADP and collagen/epinephrine cartridges) to assess the in vitro effects of a few platelet function inhibitors: Aspisol (60 microg/ml), 4-[4-[4-(aminoiminomethyl]-1-piperazinyl]-1-piperidineactetic acid, hydrochloride trihydrate (GR144053F, fibrinogen receptor antagonist, 100 nM), adenosine-3',5'-diphosphate (A3P5P, P2Y1 ADP receptor antagonist, 500 microM) and Bis[(adenosine-5'-O-phosphorodithioyl)methylene]-phosphinic acid (APTMPA, P2Y12 ADP receptor antagonist, 500 microM) on platelet function, as compared with the other commonly used diagnostic technique, a whole blood electrical aggregometry (20 microM ADP or 0.5 mM arachidonic acid). The in vitro studies were carried out on a group of 38 subjects. Whereas all the examined platelet antagonists and inhibitors almost completely blocked the 20 microM ADP- or 0.5 mM arachidonic acid-induced (in the case of acetylsalicylic acid) whole blood aggregation, only two inhibitors (Aspisol and GR144053F) remained effective in a significant prolongation of the PFA-100 occlusion time. Otherwise, using the PFA-100 system we were not able to detect the inhibitory actions of ADP receptor antagonists- P2Y1 and P2Y12. Our findings point to a limited usefulness of the PFA-100 system for the monitoring of the effectiveness of ADP receptor antagonists. The outcomes of this study show that platelet aggregometry in whole blood is characterised by the highest sensitivity in the monitoring of the investigated blood platelet inhibitors. |
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Authors:
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Jacek Golanski; Justyna Pluta; Janina Baraniak; Cezary Watala |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Clinical chemistry and laboratory medicine : CCLM / FESCC Volume: 42 ISSN: 1434-6621 ISO Abbreviation: Clin. Chem. Lab. Med. Publication Date: 2004 Jan |
Date Detail:
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Created Date: 2004-04-05 Completed Date: 2004-10-08 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9806306 Medline TA: Clin Chem Lab Med Country: Germany |
Other Details:
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Languages: eng Pagination: 25-9 Citation Subset: IM |
Affiliation:
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Department of Haemostasis and Haemostatic Disorders, Medical University of Lodz, Lodz, Poland. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Diphosphate* Adult Blood Platelets / cytology, drug effects, metabolism Collagen* Epinephrine* Female Hematologic Tests / instrumentation*, methods* Humans Male Platelet Aggregation / drug effects Platelet Aggregation Inhibitors / pharmacology* Receptors, Purinergic P2 / antagonists & inhibitors*, metabolism Whole Blood Coagulation Time |
| Chemical | |
Reg. No./Substance:
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0/Platelet Aggregation Inhibitors; 0/Receptors, Purinergic P2; 51-43-4/Epinephrine; 58-64-0/Adenosine Diphosphate; 9007-34-5/Collagen |
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