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Limited inhibitory effects of non-steroidal antiinflammatory drugs on in vitro osteogenic differentiation in canine cells.
MedLine Citation:
PMID:  24059095     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
Cyclooxygenase (COX)-2 participates essentially in bone healing, demonstrated by COX-2 knockout mice that showed delayed fracture repair. Considerable controversy still exists on inhibitory effects of COX-2 inhibitors on bone healing in clinical cases. To assess stage-dependent effects of short-term treatment of COX-2 inhibitors on osteogenic differentiation, a canine POS osteosarcoma cell line which spontaneously differentiates into osteoblastic cell was exposed to COX-2 inhibitors such as carprofen and meloxicam for 72 hours during three different stages of osteoblast differentiation, including day 0 to 3 (pre-osteoblastic stage), day 4 to 7 (transitional stage) and day 8 to 11 (mature osteoblastic stage). As osteogenic markers, expression of alkaline phosphatase (ALP) was estimated by analysis of mRNA expression, enzymatic activity and ALP staining, and expression of osteocalcin was estimated by analysis of mRNA expression after the drug treatments. Calcified matrix formation was finally observed by von Kossa staining on day 14. Expressions of ALP showed no significant suppression by carprofen and meloxicam during all three stages. However, expressions of osteocalcin mRNA and non-calcified nodule formations were delayed by carprofen and meloxicam during transitional stage. Nevertheless, fully calcified nodule formation was observed in all experimental groups during post-medication period. These results indicate that short-term treatment of carprofen and meloxicam would reversibly suppress the differentiation of osteoblasts.
Authors:
Namgil Oh; Takafumi Sunaga; Hiroki Yamazaki; Kenji Hosoya; Satoshi Takagi; Masahiro Okumura
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Japanese journal of veterinary research     Volume:  61     ISSN:  0047-1917     ISO Abbreviation:  Jpn. J. Vet. Res.     Publication Date:  2013 Aug 
Date Detail:
Created Date:  2013-09-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0376567     Medline TA:  Jpn J Vet Res     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  97-107     Citation Subset:  IM    
Affiliation:
Laboratory of Veterinary Surgery, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan. ngism@hotmail.com
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