Document Detail

Limited expression of Fas and Fas ligand in fetal nucleated erythrocytes isolated from first trimester maternal blood.
MedLine Citation:
PMID:  12478636     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: Intact fetal cells isolated from maternal blood can be used for non-invasive gender determination and genetic diagnosis. Recent studies demonstrating a large amount of cell-free fetal DNA in maternal plasma suggest that the circulating fetal DNA may result from fetal cells undergoing apoptosis. In the present study we evaluated the potential role of Fas and Fas ligand (FasL) cell surface expression with respect to apoptosis induction in fetal cells isolated from maternal blood. METHODS: We flow sorted candidate fetal cells that were gamma chain-positive and Fas- or FasL-positive or -negative, and subsequently analysed them by fluorescence in situ hybridization (FISH) analysis using X and Y chromosome-specific probes. RESULTS: Among all gamma hemoglobin-positive cells, there was a significant difference in the percent of cells expressing Fas versus FasL (4.4 and 12.3, respectively). We found no significant correlation between the total number of fetal nucleated red blood cells (NRBCs) and gestational age or the presence of Fas- and FasL-positive cells. From approximately 7 ml of maternal peripheral blood, most of the confirmed fetal (XY) cells were found in the Fas- and FasL-negative sorted population; the average numbers were 12.8 and 15.7, respectively. CONCLUSION: We conclude that fetal NRBCs express FasL more than Fas, although most fetal NRBCs in first trimester maternal blood samples do not express Fas or FasL. This suggests the absence of a functional Fas/FasL apoptotic system in fetal NRBCs, and that programmed cell death in these cells, which may lead to circulating fetal DNA in maternal plasma, probably occurs by another pathway.
Satoshi Sohda; Osamu Samura; Kirby L Johnson; Vincent M Falco; R Sarah Elmes; Diana W Bianchi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Prenatal diagnosis     Volume:  22     ISSN:  0197-3851     ISO Abbreviation:  Prenat. Diagn.     Publication Date:  2002 Dec 
Date Detail:
Created Date:  2002-12-12     Completed Date:  2003-06-03     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8106540     Medline TA:  Prenat Diagn     Country:  England    
Other Details:
Languages:  eng     Pagination:  1213-8     Citation Subset:  IM    
Copyright Information:
Copyright 2002 John Wiley & Sons, Ltd.
Division of Genetics, Department of Pediatrics, New England Medical Center, Tufts University School of Medicine, Boston, MA 02111, USA.
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MeSH Terms
Antigens, CD95 / metabolism*
Apoptosis / physiology
DNA / analysis
DNA Fragmentation
Erythroblasts / metabolism*
Fas Ligand Protein
Fetal Blood / cytology*,  metabolism
Fetomaternal Transfusion
Flow Cytometry
In Situ Hybridization, Fluorescence
Membrane Glycoproteins / metabolism*
Pregnancy / blood*
Pregnancy Trimester, First
Grant Support
Reg. No./Substance:
0/Antigens, CD95; 0/FASLG protein, human; 0/Fas Ligand Protein; 0/Membrane Glycoproteins; 9007-49-2/DNA

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