Document Detail

Limited T-cell receptor diversity in liver-infiltrating lymphocytes from patients with primary biliary cirrhosis.
MedLine Citation:
PMID:  8240664     Owner:  NLM     Status:  MEDLINE    
Primary biliary cirrhosis is associated with the presence of high-titer anti-mitochondrial autoantibodies as well as T-cell infiltration of the liver, suggesting the involvement of autoimmune mechanisms. We have studied here the sequences of T-cell receptor alpha and beta chains expressed by T-cell clones derived from liver-infiltrating lymphocytes of two patients with primary biliary cirrhosis. Among the eight clones studied from the first patient, four expressed the same member of the V beta 6 subfamily, associated with either V alpha 4 (three clones) or V alpha 21 (one clone) gene segment. Two other clones expressed an identical V beta 12 transcript, and two in-frame alpha chain transcripts, involving V alpha 2 and V alpha 7 gene segments. From the second patient, eight out of the nine clones were found to rearrange V beta 17-J beta 2.1 and V alpha 3 gene segments. The remaining clone expressed distinct T-cell receptor chains, involving V beta 9 and V alpha 11 gene segments. As deduced from the analysis of their junctional regions, the eight T-cell clones expressing V beta 17/V alpha 3 gene segments derived from only three different T cells. Furthermore, conserved amino acid motifs were found to be encoded in both the alpha and the beta-chain junctional regions. Together, these data show a local amplification of unique T lymphocytes in both patients. The use of identical V beta J beta and V alpha gene segments with similar junctional sequences by three different cells, evidenced in one of the two cases, strengthens the view that liver-infiltrating T lymphocytes are selected locally by autoantigens in PBC.
A Diu; U Moebius; L Ferradini; C Genevée; S Roman-Roman; M Claudon; D Delorme; S Meuer; T Hercend; F Praz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of autoimmunity     Volume:  6     ISSN:  0896-8411     ISO Abbreviation:  J. Autoimmun.     Publication Date:  1993 Oct 
Date Detail:
Created Date:  1993-12-30     Completed Date:  1993-12-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8812164     Medline TA:  J Autoimmun     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  611-9     Citation Subset:  IM    
Département d'Immunologie, Roussel-Uclaf, Romainville, France.
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MeSH Terms
Amino Acid Sequence
Base Sequence
Liver Cirrhosis, Biliary / immunology*
Middle Aged
Molecular Sequence Data
Receptors, Antigen, T-Cell, alpha-beta / physiology*
Reg. No./Substance:
0/Receptors, Antigen, T-Cell, alpha-beta

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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