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Limited fatty infiltration due to apoptosis in human degenerated temporomandibular joint disks: an immunohistochemical study.
MedLine Citation:
PMID:  20856040     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
In this study, we hypothesized that caspase 3, which plays a central role in the execution phase of cell apoptosis, could be involved in limiting fatty degeneration of the temporomandibular joint (TMJ) disks and therefore inhibit the TMJ disk tissue from completely degenerating into fatty tissue. Therefore, caspase 3 immunohistochemical expression in human TMJ degenerated disks was studied. Fifty-nine degenerated TMJ disks were stained with Harry's hematoxylin, and they were then examined with light microscopy to detect any pathologic changes typical of fatty degeneration. Sections from the same TMJ disk were immunostained also by a polyclonal anti-caspase 3 antibody. On morphologic observations, 11 disks of 59 degenerated ones also presented a fatty infiltration. Immunostaining with caspase 3 antibody was detected on adipocytes in the cytoplasm as well as the nuclei. Our results sustain the hypothesis that fatty degeneration is limited by apoptosis, being adipocytes immunolabeled by caspase 3 antibody. Hence, apart from the several factors that can trigger degeneration changes in TMJ disk, their appearance, spread, and permanence, at least for fatty degeneration, seem to be influenced by apoptosis.
Authors:
Rosalia Leonardi; Maria Rita Migliore; Luis Eduardo Almeida; Paula C Trevilatto; Carla Loreto
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of craniofacial surgery     Volume:  21     ISSN:  1536-3732     ISO Abbreviation:  J Craniofac Surg     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-09-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9010410     Medline TA:  J Craniofac Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1508-11     Citation Subset:  D    
Affiliation:
Department of Dentistry, Faculty of Dentistry, University of Catania, Policlinico Universitario, Italy. rleonard@unict.it
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