| Limb blood flow during exercise is dependent on nitric oxide. | |
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MedLine Citation:
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PMID: 9711943 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: We have recently reported that hypercholesterolemia reduces aerobic exercise capacity in mice and that this is associated with a reduced endothelium-dependent vasodilator function, endothelium-derived nitric oxide (EDNO) production, and urinary nitrate excretion. These findings led us to test the hypothesis that EDNO production contributes significantly to limb blood flow during exercise and to determine whether loss of EDNO production is responsible for the decline in exercise capacity observed in hypercholesterolemia. METHODS AND RESULTS: Twelve-week-old wild-type (E+; n=9) and apoE-deficient (E-; n=9) C57BL/6J mice were treadmill-tested to measure indices defining exercise capacity on a metabolic chamber-enclosed treadmill capable of measuring oxygen uptake and carbon dioxide excretion. Urine was collected before and after treadmill exercise for determination of vascular NO production assessed by urinary nitrate excretion. The wild-type mice were then given nitro-L-arginine (E+LNA) in the drinking water (6 mg/dL) for 4 days before undergoing a second treadmill testing and urinary nitrate measurement. An additional set of 12-week-old wild-type mice was divided into 2 groups: 1 receiving regular water (E+; n=8) and 1 administered LNA for 4 days (E+LNA; n=8). These mice, along with an additional set of E mice (n=8), underwent treadmill testing to determine maximal oxygen uptake (VO2max). The mice were then cannulated such that the tip of the tubing was positioned in the ascending aorta. Fluorescent microspheres (20000) were infused into the carotid cannula while the mice were sedentary and again while approaching VO2max. When the mice were euthanized, the running muscles were collected and fluorescence intensity was measured to determine the peak-exercise redistribution of blood flow to the running muscles (expressed as percentage of total cardiac output, %COrm) during both states. Both E+LNA and E- mice demonstrated a markedly reduced postexercise urinary nitrate excretion, aerobic capacity, and %COrm at VO2max compared with E+. CONCLUSIONS: EDNO contributes significantly to limb blood flow during exercise. Conditions that reduce EDNO production disturb the hyperemic response to exercise, resulting in a reduced exercise capacity. |
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Authors:
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A J Maxwell; E Schauble; D Bernstein; J P Cooke |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Circulation Volume: 98 ISSN: 0009-7322 ISO Abbreviation: Circulation Publication Date: 1998 Jul |
Date Detail:
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Created Date: 1998-09-11 Completed Date: 1998-09-11 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0147763 Medline TA: Circulation Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 369-74 Citation Subset: AIM; IM |
Affiliation:
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Section of Vascular Medicine, Stanford University, Calif 94305-5246, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apolipoproteins E / deficiency Enzyme Inhibitors / pharmacology Extremities / blood supply* Female Hypercholesterolemia / metabolism, physiopathology Hyperemia / etiology, physiopathology Mice Mice, Inbred C57BL Motor Activity / physiology* Nitrates / urine Nitric Oxide / physiology* Nitroarginine / pharmacology Physical Endurance / physiology Regional Blood Flow / physiology |
| Grant Support | |
ID/Acronym/Agency:
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1RO1-HL-58638/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Apolipoproteins E; 0/Enzyme Inhibitors; 0/Nitrates; 10102-43-9/Nitric Oxide; 2149-70-4/Nitroarginine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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