Document Detail

Lim1 is essential for the correct laminar positioning of retinal horizontal cells.
MedLine Citation:
PMID:  18094249     Owner:  NLM     Status:  MEDLINE    
Although much is known about the transcriptional regulation that coordinates retinal cell fate determination, very little is known about the developmental processes that establish the characteristic laminar architecture of the retina, in particular, the specification of neuronal positioning. The LIM class homeodomain transcription factor Lim1 (Lhx1) is expressed in postmitotic, differentiating, and mature retinal horizontal cells. We show that conditional ablation of Lim1 results in the ectopic localization of horizontal cells to inner aspects of the inner nuclear layer, among the retinal amacrine cells. The ectopic cells maintain a molecular phenotype consistent with horizontal cell identity; however, these neurons adopt a unique morphology more reminiscent of amacrine cells, including a dendritic arbor positioned within the inner plexiform layer. All other retinal cell populations appear unaltered. Our data suggest a model whereby Lim1 lies downstream of horizontal cell fate determination factors and functions cell autonomously to instruct differentiating horizontal cells to the appropriate laminar position in the developing retina. This study is the first to describe a cell type-specific genetic program that is essential for targeting a discrete retinal neuron population to the proper lamina.
Ross A Poché; Kin Ming Kwan; Mary A Raven; Yasuhide Furuta; Benjamin E Reese; Richard R Behringer
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of neuroscience : the official journal of the Society for Neuroscience     Volume:  27     ISSN:  1529-2401     ISO Abbreviation:  J. Neurosci.     Publication Date:  2007 Dec 
Date Detail:
Created Date:  2007-12-20     Completed Date:  2008-01-14     Revised Date:  2008-05-08    
Medline Journal Info:
Nlm Unique ID:  8102140     Medline TA:  J Neurosci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  14099-107     Citation Subset:  IM    
Program in Developmental Biology, Baylor College of Medicine, Houston, Texas 77030, USA.
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MeSH Terms
Amino Acid Sequence
Cell Differentiation / genetics
Cell Movement / physiology
Gene Expression Regulation, Developmental / physiology
Homeodomain Proteins / biosynthesis,  genetics,  physiology*
Mice, Inbred C57BL
Mice, Knockout
Mice, Transgenic
Molecular Sequence Data
Retinal Horizontal Cells / cytology,  embryology*,  metabolism*
Grant Support
Reg. No./Substance:
0/Homeodomain Proteins; 0/Lhx1 protein, mouse
Comment In:
J Neurosci. 2008 Apr 9;28(15):3835-6   [PMID:  18400881 ]

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